研究活细胞中的膜蛋白动力学。

Investigating membrane protein dynamics in living cells.

作者信息

Bates Ian R, Wiseman Paul W, Hanrahan John W

机构信息

Department of Physiology, McGill University, 3655 Promenade Sir William Osler, Montréal, QC H3G 1Y6, Canada.

出版信息

Biochem Cell Biol. 2006 Dec;84(6):825-31. doi: 10.1139/o06-189.

DOI:10.1139/o06-189

PMID:17215870

Abstract

Live cell imaging is a powerful tool for understanding the function and regulation of membrane proteins. In this review, we briefly discuss 4 fluorescence-microscopy-based techniques for studying the transport dynamics of membrane proteins: fluorescence-correlation spectroscopy, image-correlation spectroscopy, fluorescence recovery after photobleaching, and single-particle and (or) molecule tracking. The advantages and limitations of each approach are illustrated using recent studies of an ion channel and cell adhesion molecules.

摘要

活细胞成像技术是了解膜蛋白功能及调控机制的有力工具。在本综述中，我们简要讨论4种基于荧光显微镜的技术，用于研究膜蛋白的转运动力学：荧光相关光谱技术、图像相关光谱技术、光漂白后荧光恢复技术以及单颗粒和（或）单分子追踪技术。我们通过最近对一种离子通道和细胞黏附分子的研究，阐述了每种方法的优缺点。