利用荧光相关光谱法和光漂白后荧光恢复技术研究植物中受体激酶的流动性。

Fluorescence Correlation Spectroscopy and Fluorescence Recovery After Photobleaching to study receptor kinase mobility in planta.

作者信息

Kwaaitaal Mark, Schor Marieke, Hink Mark A, Visser Antonie J W G, de Vries Sacco C

机构信息

Laboratory of Biochemistry, Wageningen University, Wageningen, The Netherlands.

出版信息

Methods Mol Biol. 2011;779:225-42. doi: 10.1007/978-1-61779-264-9_13.

DOI:10.1007/978-1-61779-264-9_13

PMID:21837570

Abstract

Plasma-membrane-localized receptor kinases are essential for cell-cell communication and as sensors for the extracellular environment. Receptor function is dependent on their distribution in the membrane and interaction with other proteins that are either membrane-localized, present in the cytoplasm, or in the extracellular space. The organized distribution and mobility of receptor kinases is, therefore, thought to regulate the efficiency of downstream signaling. This chapter describes two methods to study receptor mobility in the plasma membrane. Fluorescence Correlation Spectroscopy (FCS) and Fluorescence Recovery After Photobleaching (FRAP). Especially, the combination of FRAP and FCS provides a better insight into plasma membrane receptor mobility.

摘要

质膜定位的受体激酶对于细胞间通讯至关重要，并且作为细胞外环境的传感器。受体功能取决于它们在膜中的分布以及与其他蛋白质的相互作用，这些蛋白质要么定位在膜上，存在于细胞质中，要么存在于细胞外空间。因此，受体激酶的有序分布和流动性被认为可以调节下游信号传导的效率。本章描述了两种研究质膜中受体流动性的方法。荧光相关光谱法（FCS）和光漂白后荧光恢复法（FRAP）。特别是，FRAP和FCS的结合能更好地洞察质膜受体的流动性。