Mäentausta O, Sormunen R, Isomaa V, Lehto V P, Jouppila P, Vihko R
Biocenter, University of Oulu, Finland.
Lab Invest. 1991 Nov;65(5):582-7.
Immunoaffinity-purified polyclonal anti-rabbit antibody against human placental 17 beta-hydroxysteroid dehydrogenase (17HSD) was used to localize 17HSD in frozen sections of 21 human endometrial tissue specimens, taken at different stages of the menstrual cycle, and in the human placenta. The presence and distribution of estrogen and progesterone receptors were also analyzed in endometrial specimens using commercial immunohistochemical techniques. In addition, 17HSD was localized by immunoelectron microscopy in the endometrium and the placenta. In the endometrium, immunostaining of 17HSD appeared in the cytoplasm of surface epithelial and gland cells during the early and midluteal phase. During the late luteal phase, it gradually disappeared. No immunostaining was observed in the endometrium during the follicular phase of the menstrual cycle. The changes in staining intensity of 17HSD were associated with changes in the concentration of serum progesterone as judged by radioimmunoassay. An apparent inverse correlation between 17HSD expression and the concentrations of estrogen and progesterone receptors was observed. These results strongly support the concept that progesterone induces an increase in the amount of 17HSD, in the glandular and surface epithelial cells of the human endometrium. In the human term placenta, 17HSD immunostaining was detected exclusively in the cytoplasm of syncytiotrophoblasts. In immunoelectron microscopic studies of the endometrium and placenta, 17HSD staining was observed in the cytoplasm and it was associated with cytoplasmic membranes unrelated to the endoplasmic reticulum.
使用免疫亲和纯化的抗人胎盘17β-羟类固醇脱氢酶（17HSD）多克隆抗兔抗体，对取自月经周期不同阶段的21份人子宫内膜组织标本的冰冻切片以及人胎盘中的17HSD进行定位。还使用商业免疫组织化学技术分析了子宫内膜标本中雌激素和孕激素受体的存在与分布。此外，通过免疫电子显微镜对子宫内膜和胎盘中的17HSD进行定位。在子宫内膜中，17HSD的免疫染色在黄体早期和中期出现在表面上皮细胞和腺细胞的细胞质中。在黄体晚期，它逐渐消失。在月经周期的卵泡期，子宫内膜中未观察到免疫染色。通过放射免疫测定判断，17HSD染色强度的变化与血清孕酮浓度的变化相关。观察到17HSD表达与雌激素和孕激素受体浓度之间存在明显的负相关。这些结果有力地支持了孕酮可诱导人子宫内膜腺细胞和表面上皮细胞中17HSD量增加的观点。在足月人胎盘中，仅在合体滋养层细胞的细胞质中检测到17HSD免疫染色。在子宫内膜和胎盘的免疫电子显微镜研究中，在细胞质中观察到17HSD染色，并且它与不相关的内质网的细胞质膜有关。
