Ozaki Tomoya, Takahashi Kentaro, Kanasaki Haruhiko, Miyazaki Kohji
Department of Obstetrics and Gynecology, Seirei Hamamatsu General Hospital, Hamamatsu, Japan.
Am J Obstet Gynecol. 2006 Nov;195(5):1343-50. doi: 10.1016/j.ajog.2006.05.014. Epub 2006 Jun 21.
The purpose of this study was to investigate the fluctuation of expression and activation of mitogen-activated protein kinase in normal human endometrium throughout the menstrual cycle.
Thirty-three normal endometrial tissues were obtained from fertile women who had undergone hysterectomies for reasons other than endometrial disease. Extracellular signal-regulated kinase, -1, and -2 expression were studied by immunohistochemistry. Moreover, extracellular signal-regulated kinase activity was analyzed by gel kinase assay.
Western blotting analysis with anti-pan-extracellular signal-regulated kinase antibody mainly demonstrated an immunoreactive band of 42 kd that corresponded to extracellular signal-regulated kinase 2 in the endometrium. The expression of extracellular signal-regulated kinase 2 tended to increase in the secretory phase. Immunohistochemical analysis for extracellular signal-regulated kinase 1 in endometrial sections revealed a weak staining of glands and almost no staining of stromal cells. Immunohistochemical analysis for extracellular signal-regulated kinase 2 in endometrial sections revealed a distinct staining of glands in both proliferative and secretory phases and a weak staining of stromal cells. Although the intensity of staining for extracellular signal-regulated kinase 2 in stromal cells did not change during the secretory phase, in the glands the extracellular signal-regulated kinase 2 was highly stained in the mid-to-late secretory phase. In gel kinase assay revealed that extracellular signal-regulated kinase activity was increased significantly in the mid-to-late secretory phase.
Expression and activation of extracellular signal-regulated kinase in the human endometrium was increased particularly during the secretory phase. We suggest that fluctuation of extracellular signal-regulated kinase in the human endometrium may be induced by ovarian steroid hormones.
本研究旨在探讨正常人子宫内膜在整个月经周期中丝裂原活化蛋白激酶表达及激活的波动情况。
从因非子宫内膜疾病行子宫切除术的有生育能力的妇女中获取33份正常子宫内膜组织。采用免疫组织化学方法研究细胞外信号调节激酶-1和-2的表达。此外，通过凝胶激酶分析检测细胞外信号调节激酶的活性。
用抗泛细胞外信号调节激酶抗体进行的蛋白质印迹分析主要显示出一条42kd的免疫反应带，对应于子宫内膜中的细胞外信号调节激酶2。细胞外信号调节激酶2的表达在分泌期有增加趋势。对子宫内膜切片中细胞外信号调节激酶1的免疫组织化学分析显示腺体染色较弱，基质细胞几乎无染色。对子宫内膜切片中细胞外信号调节激酶2的免疫组织化学分析显示，在增殖期和分泌期腺体均有明显染色，基质细胞染色较弱。虽然基质细胞中细胞外信号调节激酶2的染色强度在分泌期没有变化，但在腺体中，细胞外信号调节激酶2在分泌中期至晚期染色强烈。凝胶激酶分析显示，细胞外信号调节激酶活性在分泌中期至晚期显著增加。
人子宫内膜中细胞外信号调节激酶的表达及激活在分泌期尤其增加。我们认为人子宫内膜中细胞外信号调节激酶的波动可能由卵巢甾体激素诱导。
