妊娠小鼠子宫内膜中颗粒状和非颗粒状的蜕膜催乳素相关蛋白阳性蜕膜细胞。

Granulated and non-granulated decidual prolactin-related protein-positive decidual cells in the pregnant mouse endometrium.

作者信息

Candeloro Luciane, Zorn Telma M T

机构信息

Laboratory of Biology of Reproduction and Extracellular Matrix, Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.

出版信息

Am J Reprod Immunol. 2007 Feb;57(2):122-32. doi: 10.1111/j.1600-0897.2006.00452.x.

DOI:10.1111/j.1600-0897.2006.00452.x

PMID:17217366

Abstract

PROBLEM

Identification of the cell types responsible for the synthesis of decidual prolactin-related protein (dPRP) in the pregnant mouse endometrium.

METHOD OF STUDY

Histochemistry and immunocytochemistry were used to determine peri-implantation dPRP and perlecan distribution in the mouse uterus.

RESULTS

We identified dPRP in pre-decidual and mature decidual cells from days 5 to 12 of pregnancy. On day 8, dPRP immunoreactivity was detected within cytoplasmic granules of a specific population of granulated decidual cells (GDCs). In mesometrial decidual cells, weak immunoreactivity was seen from days 7 to 14. Between days 11 and 14, dPRP was found in cytoplasm and in the extracellular matrix surrounding islands of spongiotrophoblast. Perlecan, a heparan sulfate proteoglycan, was co-localized with dPRP.

CONCLUSION

GDCs are a putative source of dPRP in pregnant mice. Co-localization of perlecan with dPRP suggests that the former acts as a dPRP reservoir and facilitates its paracrine effect in developing placental tissues.

摘要

问题

确定妊娠小鼠子宫内膜中负责合成蜕膜催乳素相关蛋白（dPRP）的细胞类型。

研究方法

采用组织化学和免疫细胞化学方法来确定着床期小鼠子宫中dPRP和基底膜聚糖的分布。

结果

我们在妊娠第5至12天的前蜕膜细胞和成熟蜕膜细胞中鉴定出了dPRP。在第8天，在特定群体的颗粒状蜕膜细胞（GDCs）的细胞质颗粒中检测到dPRP免疫反应性。在子宫系膜蜕膜细胞中，从第7天到第14天可见弱阳性反应。在第11天至14天之间，在海绵滋养层细胞岛周围的细胞质和细胞外基质中发现了dPRP。基底膜聚糖，一种硫酸乙酰肝素蛋白聚糖，与dPRP共定位。