San Martin S, Soto-Suazo M, Zorn T M T
Laboratório de Biologia da Reproducao e da Matriz Extracelular, Departamento de Histologia e Embriologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, Brazil.
Am J Reprod Immunol. 2004 Jul;52(1):53-9. doi: 10.1111/j.1600-0897.2004.00182.x.
During early pregnancy in mice, there is recruitment of specific immune cells, remodeling of the endometrium, cell differentiation and synthesis of new molecules.
Immunohistochemistry was used to determine the distribution of perlecan and syndecan-4 in the uteri before and after embryo implantation.
During pre-implantation, perlecan was identified in basement membranes and extracellular spaces of the endometrial stroma. In contrast, expression of syndecan-4 was quite weak. In the peri-implantation period, perlecan remained in the basement membranes, and it was no longer observed in the stroma and it was identified in the embryonic cells. On day 4 of pregnancy, syndecan-4 increased in the fibroblasts of the subepithelial stroma. After implantation, syndecan-4 was pronounced in pre-decidual and mature decidual cells.
The coordinate balance between the pre- and post-implantation periods suggests a role of these two molecules in the adaptive modification of the uterine microenvironment to receive and implant the embryo.
在小鼠妊娠早期，会募集特定免疫细胞、重塑子宫内膜、进行细胞分化并合成新分子。
采用免疫组织化学法测定胚胎植入前后子宫中基底膜聚糖和多功能蛋白聚糖-4的分布。
在植入前，基底膜聚糖在子宫内膜基质的基底膜和细胞外间隙中被识别。相比之下，多功能蛋白聚糖-4的表达相当微弱。在植入期，基底膜聚糖仍存在于基底膜中，在基质中不再观察到，并且在胚胎细胞中被识别。在妊娠第4天，多功能蛋白聚糖-4在子宫上皮下基质的成纤维细胞中增加。植入后，多功能蛋白聚糖-4在蜕膜前细胞和成熟蜕膜细胞中显著表达。
植入前后阶段的协调平衡表明这两种分子在子宫微环境接受和植入胚胎的适应性改变中发挥作用。
