Chang R X, Sun Q, Guo Y W, Zhu Q R, Pan Y C
Academia Sinica, Shanghai Institute of Cell Biology.
Shi Yan Sheng Wu Xue Bao. 1991 Sep;24(3):189-201.
By indirect immunofluorescence microscopy and electron microscopy, we studied the behavior of intermediate filaments during mitosis in three human epithelial cell lines, derived from normal epidermis (PcaSE-1, from a cancer patient), stratified epithelium (CNE, from nasopharyngeal carcinoma) and simple epithelium (SPC-A-1 from lung adenocarcinoma) respectively. CNE cells and SPC-A-1 cells express two different intermediate filament systems; keratin filaments and vimentin filaments, but PcaSE-1 cells only express keratin filaments. The keratin filament system in PcaSE-1 cells remained intact and encircled the developing mitotic spindle as the cells entered mitosis. In contrast, in CNE cells and SPC-A-1 cells, keratin filaments appeared to disassemble into amorphous cytoplasmic bodies during mitosis. However, their vimentin filaments remained morphologically intact throughout mitosis. We propose; (1) The disassembly of keratin filaments in mitotic epithelial cells is more or less associated with the degree of their cell malignancy rather than with the abundance of keratin filaments in interphase. (2) Intermediate filaments may be involved in the positioning and/or centering of the spindle during mitosis. (3) The possible function of vimentin filament system in CNE cells is positioning and orientation of chromosomes.
通过间接免疫荧光显微镜和电子显微镜，我们研究了三种人上皮细胞系在有丝分裂过程中中间丝的行为，这三种细胞系分别来源于正常表皮（PcaSE - 1，来自一名癌症患者）、复层上皮（CNE，来自鼻咽癌）和单层上皮（SPC - A - 1来自肺腺癌）。CNE细胞和SPC - A - 1细胞表达两种不同的中间丝系统；角蛋白丝和波形蛋白丝，但PcaSE - 1细胞只表达角蛋白丝。当PcaSE - 1细胞进入有丝分裂时，其角蛋白丝系统保持完整并环绕正在形成的有丝分裂纺锤体。相比之下，在CNE细胞和SPC - A - 1细胞中，角蛋白丝在有丝分裂期间似乎解体成无定形的细胞质体。然而，它们的波形蛋白丝在整个有丝分裂过程中在形态上保持完整。我们提出：（1）有丝分裂上皮细胞中角蛋白丝的解体或多或少与细胞恶性程度有关，而非与间期角蛋白丝的丰度有关。（2）中间丝可能参与有丝分裂期间纺锤体的定位和/或居中。（3）CNE细胞中波形蛋白丝系统的可能功能是染色体的定位和定向。
