Cai S T
Shanghai Institute of Cell Biology, Academia Sinica.
Shi Yan Sheng Wu Xue Bao. 1990 Dec;23(4):465-75.
Immunofluorescence microscopy was used to follow the rearrangement of keratin filaments and vimentin filaments during mitosis in Vero and HeLa cell lines. The experiment results showed that the three dimensional organization and structure of intermediate filaments changed drastically during mitosis. The behavior of intermediate filaments was different in these two epithelial cell lines. In mitotic Vero cells the keratin filaments and vimentin filaments maintained their filamentous structure and formed a cage around the mitotic apparatus. In mitotic HeLa cells the keratin filaments and vimentin filaments reorganized extensively and formed granular cytoplasmic bodies. The ratio of granular cytoplasmic body formation changed in different mitotic phase. The interphase intermediate filament network was reconstructed after mitosis. It is proposed that the state of intermediate filament network in these cells is cell cycle-dependent and intermediate filaments may have some skeletal role in mitosis.
利用免疫荧光显微镜观察了Vero和HeLa细胞系有丝分裂过程中角蛋白丝和波形蛋白丝的重排。实验结果表明，中间丝的三维组织和结构在有丝分裂过程中发生了剧烈变化。这两种上皮细胞系中中间丝的行为有所不同。在有丝分裂的Vero细胞中，角蛋白丝和波形蛋白丝保持其丝状结构，并在有丝分裂器周围形成一个笼子。在有丝分裂的HeLa细胞中，角蛋白丝和波形蛋白丝广泛重组并形成颗粒状细胞质体。颗粒状细胞质体形成的比例在不同的有丝分裂期有所变化。有丝分裂后间期中间丝网络得以重建。有人提出，这些细胞中中间丝网络的状态依赖于细胞周期，中间丝在有丝分裂中可能具有某种骨架作用。
