[氯沙坦对心力衰竭兔肌浆网Ca2+处理蛋白的影响]

[Effect of losartan on sarcoplasmic reticulum Ca2+ handing proteins in heart failure rabbit].

作者信息

Yao Yan, Huang Cong-xin, Chen Gao, Xu Lin

机构信息

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China.

出版信息

Zhonghua Xin Xue Guan Bing Za Zhi. 2006 Sep;34(9):793-6.

PMID:17217685

Abstract

OBJECTIVE

To investigate the effects of losartan on mRNA expression of myocardial sarcoplasmic reticulum calcium handling proteins (SERCA2, RyR2 and PLB) and the role of which in prevention of chronic heart failure in rabbit.

METHODS

After chronic heart failure was induced by ligation of the left anterior descending artery in rabbits, the animals were treated with losartan. At 8 weeks after ligation, left ventricular function, hemodynamic parameters, and SERCA2, RyR2, PLB mRNA expressions were observed.

RESULTS

Compared with the control group (group C), LVEDP in the infarcted group (group I) increased (P < 0.01), while +dp/dt(max) and -dp/dt(max) decreased significantly (P < 0.01). LVEDP was lower but +dp/dt(max) and -dp/dt(max) significantly higher in the losartan treated group (group L) than those in group I (P < 0.05). SERCA2, RyR2, and PLB mRNA expressions in group I were remarkably lower than those in group L (P < 0.01) and group C (P < 0.01), respectively.

CONCLUSION

Losartan can improve cardiac function, probably owing to its upregulating mRNA expressions of myocardial sarcoplasmic reticulum Ca(2+) handling proteins (RyR2, SERCA2 and PLB) in the prevention of heart failure.

摘要

目的

探讨氯沙坦对兔心肌肌浆网钙处理蛋白（SERCA2、RyR2和PLB）mRNA表达的影响及其在预防兔慢性心力衰竭中的作用。

方法

通过结扎兔左冠状动脉前降支诱导慢性心力衰竭，然后用氯沙坦对动物进行治疗。结扎后8周，观察左心室功能、血流动力学参数以及SERCA2、RyR2、PLB mRNA表达。

结果

与对照组（C组）相比，梗死组（I组）左室舒张末压升高（P<0.01），而+dp/dt(max)和-dp/dt(max)显著降低（P<0.01）。氯沙坦治疗组（L组）左室舒张末压低于I组，但+dp/dt(max)和-dp/dt(max)显著高于I组（P<0.05）。I组中SERCA2、RyR2和PLB mRNA表达分别显著低于L组（P<0.01）和C组（P<0.01）。