Zhang Yan, Lin Guo-sheng, Bao Ming-wei, Wu Xin-ying, Wang Che, Yang Bo
Department of Cardiology, Renmin Hospital of Wuhan University, Cardiovascular Research Institute, Wuhan 430060, China.
Zhonghua Xin Xue Guan Bing Za Zhi. 2010 Apr;38(4):369-73.
To investigate the effects of curcumin on sarcoplasmic reticulum Ca2+-ATPase in heart failure rabbits.
Rabbit heart failure model was made with aortic regurgitation and abdominal aorta constriction and 40 rabbits were randomly divided into 4 groups including: (1) heart failure treated with curcumin; (2) heart failure treated with placebo; (3) healthy control treated with curcumin and (4) healthy control treated with placebo. All rabbits were administrated with curcumin capsules or placebo capsules 100 mg x kg(-1) x d(-1), respectively. All groups were sacrificed after eight weeks. Myocardial ultrastructural organization was detected by transmission electron microscope. RT-PCR and Western blot were used to measure the expression of sarcoplasmic reticulum Ca2+-ATPase in mRNA and protein levels, respectively. Malachite green colorimetric assay was used to evaluate the activity of sarcoplasmic reticulum Ca2+-ATPase.
All detected parameters were similar between control curcumin group and control placebo group. Compared with the control groups (Groups 3 and 4), the heart/body weight ratio was significantly increased in the heart failure-curcumin group (Group 1) and the heart failure-placebo group (Group 2, all P < 0.05), but the ratio was significantly lower in heart failure-curcumin group than in heart failure-placebo group (P < 0.05). The degree of heart failure was decreased by curcumin. Activity and mRNA and protein expression for sarcoplasmic reticulum Ca2+-ATPase were significantly reduced in the heart failure-placebo group and which could be significantly attenuated by curcumin (all P < 0.05).
Curcumin could improve cardiac function via upregulating the expression of sarcoplasmic reticulum Ca2+-ATPase in this model.
研究姜黄素对心力衰竭家兔肌浆网Ca2+-ATP酶的影响。
采用主动脉瓣反流加腹主动脉缩窄法制备家兔心力衰竭模型，40只家兔随机分为4组：（1）姜黄素治疗心力衰竭组；（2）安慰剂治疗心力衰竭组；（3）姜黄素治疗健康对照组；（4）安慰剂治疗健康对照组。所有家兔分别给予姜黄素胶囊或安慰剂胶囊，剂量均为100 mg·kg-1·d-1。8周后处死所有家兔。用透射电子显微镜检测心肌超微结构。分别用RT-PCR和Western blot法检测肌浆网Ca2+-ATP酶在mRNA和蛋白水平的表达。采用孔雀石绿比色法评估肌浆网Ca2+-ATP酶的活性。
姜黄素对照组和安慰剂对照组所有检测指标相似。与对照组（第3组和第4组）相比，心力衰竭姜黄素组（第1组）和心力衰竭安慰剂组（第2组）的心/体重比值均显著升高（均P<0.05），但心力衰竭姜黄素组的心/体重比值显著低于心力衰竭安慰剂组（P<0.05）。姜黄素可减轻心力衰竭程度。心力衰竭安慰剂组肌浆网Ca2+-ATP酶的活性及mRNA和蛋白表达均显著降低，而姜黄素可显著减弱这种降低（均P<0.05）。
在该模型中，姜黄素可通过上调肌浆网Ca2+-ATP酶的表达改善心脏功能。
