Liu Ying, Wang Li-li
Department of Obstetrics and Gynecology, Guangdong Provincial People's Hospital, Guangzhou, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2008 Aug;28(9):1727-30.
To evaluate the effect of mifepristone in reversing multidrug resistance(MDR) and modulating glucosylceramide synthase (GCS) mRNA expression in human ovarian cancer COC(1)/DDP cells.
MDR cell line COC(1)/DDP was treated with mifepristone at different concentrations. The alterations in the chemosensitivity of the cells to cisplatin (DDP) were evaluated by MTT assay. GCS mRNA expression in COC(1)/DDP cells were detected using RT-PCR before and after mifepristone treatment.
The expression level of GCS mRNA was 1.1792 in COC(1)/DDP cells, significantly higher than that in COC(1) cells (0.2836). Mifepristone at 1.25-10 micromol/L increased the sensitivity of COC(1)/DDP cells to cisplatin, and inhibited GCS expression at the mRNA level, showing concentration-dependent modulation of MDR and gene expression in the cells.
Mifepristone can dose-dependently lower cisplatin resistance of COC(1)/DDP cells, the mechanism of which involves inhibition of GCS expression.
评估米非司酮对人卵巢癌COC(1)/DDP细胞多药耐药性（MDR）的逆转作用及对葡糖神经酰胺合酶（GCS）mRNA表达的调节作用。
用不同浓度的米非司酮处理多药耐药细胞系COC(1)/DDP。采用MTT法评估细胞对顺铂（DDP）化疗敏感性的变化。在米非司酮处理前后，用RT-PCR检测COC(1)/DDP细胞中GCS mRNA的表达。
COC(1)/DDP细胞中GCS mRNA表达水平为1.1792，显著高于COC(1)细胞（0.2836）。1.25 - 10 μmol/L的米非司酮增加了COC(1)/DDP细胞对顺铂的敏感性，并在mRNA水平抑制了GCS表达，显示出对细胞多药耐药性和基因表达的浓度依赖性调节。
米非司酮可剂量依赖性降低COC(1)/DDP细胞对顺铂的耐药性，其机制涉及抑制GCS表达。
