Hisada T, Ishizuka T, Kurosawa M
First Department of Internal Medicine, Gunma University School of Medicine.
Arerugi. 1991 May;40(5):555-8.
Rat peritoneal mast cells purified on a Percoll gradient were challenged with substance P and effect of phospholipase A2 inhibitor ONO-RS-082 on substance P-induced histamine release from the cells were investigated. Substance P at the concentration of 10(-5) M caused a significant histamine release and the amount of histamine release reached to its submaximum at 1 min after the challenge and then slowly increased. ONO-RS-082 inhibited the substance P-induced histamine release in a concentration-dependent manner at the concentration from 10(-6) to 10(-4) M, suggesting that phospholipase A2 may play some roles in the process of substance P-induced histamine release from rat peritoneal mast cells.
用Percoll梯度纯化的大鼠腹膜肥大细胞用P物质刺激，并研究磷脂酶A2抑制剂ONO-RS-082对P物质诱导的细胞组胺释放的影响。浓度为10(-5) M的P物质引起显著的组胺释放，组胺释放量在刺激后1分钟达到次最大值，然后缓慢增加。ONO-RS-082在10(-6)至10(-4) M的浓度范围内以浓度依赖的方式抑制P物质诱导的组胺释放，这表明磷脂酶A2可能在P物质诱导大鼠腹膜肥大细胞组胺释放的过程中发挥某些作用。
