Winefield Robert D, Hilario Elena, Beever Ross E, Haverkamp Richard G, Templeton Matthew D
The Horticulture and Food Research Institute of New Zealand Ltd, Private Bag 92-169, Auckland, New Zealand.
Fungal Genet Biol. 2007 Apr;44(4):250-7. doi: 10.1016/j.fgb.2006.11.008. Epub 2007 Jan 10.
Homologs of the gene encoding the hydrophobin EAS from Neurospora crassa have been identified both in the other conidial species of Neurospora (N. discreta, N. intermedia, N. sitophila, and N. tetrasperma) and selected aconidial species (N. africana, N. dodgei, N. lineolata, N. pannonica, and N. terricola). Southern blot analysis indicated the presence of a single gene in all species examined. EAS-like proteins were purified from the conidial species and each was shown to be the proteolytically processed gene-product of the corresponding eas homolog. While EAS-like proteins were not detected in the aconidial species, putative eas transcripts were detected in some isolates following RT-PCR and the aerial hyphae of these species were hydrophobic. DNA sequences of the coding region of the eas homologs were amplified by PCR and cloned and sequenced from all species except N. pannonica. Phylogenetic analysis of these sequences produced two clusters, the first comprising the conidiating species N. crassa, N. intermedia, N. sitophila, and N. tetrasperma forming a closely related group with N. discreta more distant, and the second comprising the aconidial species N. africana, N. dodgei, N. lineolata forming another closely related group with N. terricola more distant.
在粗糙脉孢菌的其他分生孢子物种（离散脉孢菌、间型脉孢菌、嗜热栖热放线菌和四孢脉孢菌）以及选定的无分生孢子物种（非洲脉孢菌、多奇脉孢菌、线纹脉孢菌、潘诺尼亚脉孢菌和土栖脉孢菌）中，均已鉴定出编码粗糙脉孢菌疏水蛋白EAS的基因同源物。Southern印迹分析表明，在所检测的所有物种中均存在单个基因。从分生孢子物种中纯化出EAS样蛋白，并且每个蛋白均显示为相应eas同源物的经蛋白水解加工的基因产物。虽然在无分生孢子物种中未检测到EAS样蛋白，但在一些分离株中通过RT-PCR检测到了推定的eas转录本，并且这些物种的气生菌丝是疏水的。通过PCR扩增eas同源物编码区的DNA序列，并从除潘诺尼亚脉孢菌之外的所有物种中进行克隆和测序。对这些序列进行系统发育分析产生了两个聚类，第一个聚类包括分生孢子形成物种粗糙脉孢菌、间型脉孢菌、嗜热栖热放线菌和四孢脉孢菌，它们形成一个密切相关的组，离散脉孢菌距离较远，第二个聚类包括无分生孢子物种非洲脉孢菌、多奇脉孢菌、线纹脉孢菌，它们与土栖脉孢菌形成另一个密切相关的组，土栖脉孢菌距离较远。
