Trott J R, Apps R
Department of Physiology, School of Medical Sciences, University of Bristol, UK.
Exp Brain Res. 1991;87(1):126-40. doi: 10.1007/BF00228514.
The olivocerebellar projection to the c1, c2 and c3 zones in the paravermal cortex of lobule Vb/c has been investigated in the cat using a combined electrophysiological/neuroanatomical tracing technique. The zonal boundaries in the paravermal cortex were located by recording, on the cerebellar surface, climbing fibre field potentials evoked in response to percutaneous stimulation of one or more paws. A small (10-30 nl) injection of WGA-HRP was then made either into the centre or into the medial or lateral geographical half of a chosen zone and the resultant distribution of retrogradely labelled cells within the contralateral inferior olive was plotted. The c1 and c3 zones were each found to consist of two mediolaterally oriented 'sub-zones' which could be distinguished by their olivocerebellar input. The medial part of the c1 zone received climbing fibre input from the rostromedial part of the dorsal accessory olive (DAO) while the lateral part of the c1 zone received climbing fibre input from middle/rostral regions of the medial accessory olive (MAO). Both medial and lateral 'sub-zones' within the c3 zone were found to receive climbing fibre input from the rostral pole of DAO but, whereas there was heavy overlap between the olivary territories projecting to the medial c1 and medial c3 subzones, olivary cells projecting to the lateral part of c3 were located more rostrally within DAO. The c2 zone was found not to be divisible into mediolaterally oriented subzones and to receive climbing fibre input from a region of MAO located rostral and somewhat lateral to the region projecting to the lateral part of the c1 zone. The sub-zonal organisation of the olivocerebellar projection to the c1, c2 and c3 zones is discussed in relation to the functional properties of the different zones.
利用电生理/神经解剖示踪技术相结合的方法，在猫身上研究了橄榄小脑向小叶Vb/c蚓旁皮质的C1、C2和C3区的投射。通过在小脑表面记录对一只或多只爪子进行经皮刺激所诱发的攀爬纤维场电位，来确定蚓旁皮质的区域边界。然后将少量（10 - 30 nl）的WGA - HRP注入选定区域的中心、内侧或外侧地理半区，绘制出对侧下橄榄核内逆行标记细胞的分布情况。发现C1和C3区各自由两个沿内侧-外侧方向排列的“亚区”组成，这两个亚区可通过其橄榄小脑输入加以区分。C1区的内侧部分接受来自背侧副橄榄核（DAO）嘴内侧部分的攀爬纤维输入，而C1区的外侧部分接受来自内侧副橄榄核（MAO）中部/嘴侧区域的攀爬纤维输入。发现C3区内的内侧和外侧“亚区”均接受来自DAO嘴极的攀爬纤维输入，但是，投射到内侧C1和内侧C3亚区的橄榄区域之间存在大量重叠，而投射到C3外侧部分的橄榄细胞在DAO内的位置更靠嘴侧。发现C2区不可分为沿内侧-外侧方向排列的亚区，且接受来自MAO中一个区域的攀爬纤维输入，该区域位于投射到C1区外侧部分的区域的嘴侧且稍外侧。结合不同区域的功能特性，讨论了橄榄小脑向C1、C2和C3区投射的亚区组织情况。
