Folkesson H G, Weström B R, Pierzynowski S G, Karlsson B W
Department of Animal Physiology, University of Lund, Sweden.
J Appl Physiol (1985). 1991 Sep;71(3):1106-11. doi: 10.1152/jappl.1991.71.3.1106.
The passage of different-sized marker molecules over the lower respiratory tract into the blood circulation during pulmonary inflammation induced by dextran, endotoxin [i.e., lipopolysaccharide from Escherichia coli (LPS)], or ferritin was assessed in the rat. Bovine immunoglobulin G (BIgG, mol wt = 150,000 Da), bovine serum albumin (BSA, mol wt = 67,000 Da), and the nonapeptide 1-deaminocysteine-8-D-arginine vasopressin (dDAVP, mol wt = 1,067 Da) were used as permeability markers after intratracheal instillation. The pathophysiological indexes of a proceeding lung inflammation were increased total cell number, changed leukocyte proportions and increased total protein content obtained in bronchoalveolar lavage, and lung edema formation shown as an increased lung wet-dry weight difference. Intratracheal instillation of dextran induced a moderate neutrophil invasion into the lungs but had no effect on the passage of the different markers over the lungs (BIgG 1.8 +/- 0.6%, BSA 3.5 +/- 1.2%, dDAVP 26.1 +/- 20.7%) compared with control rats instilled with the markers alone (1.8 +/- 0.4%, 4.1 +/- 1.3%, 20.0 +/- 3.8%, respectively). Endotoxin administration resulted in markedly higher lavage cell counts and lung edema concomitantly with an increased lung passage of the markers (3.2 +/- 0.9%, 22.0 +/- 6.1%, 33.3 +/- 12.0%, respectively; P less than 0.01-P less than 0.001). The highest marker passage was obtained when the inflammation was most severe, i.e., after ferritin administration (17.6 +/- 2.3%, 60.0 +/- 6.7%, 41.6 +/- 6.9%, respectively; P less than 0.001), which resulted in markedly elevated lavage cell numbers and protein content as well as edema formation.(ABSTRACT TRUNCATED AT 250 WORDS)
在大鼠中评估了右旋糖酐、内毒素[即来自大肠杆菌（LPS）的脂多糖]或铁蛋白诱导的肺部炎症期间，不同大小的标记分子经下呼吸道进入血液循环的情况。经气管内滴注后，使用牛免疫球蛋白G（BIgG，分子量 = 150,000道尔顿）、牛血清白蛋白（BSA，分子量 = 67,000道尔顿）和九肽1-脱氨半胱氨酸-8-D-精氨酸加压素（dDAVP，分子量 = 1,067道尔顿）作为通透性标记物。进行性肺部炎症的病理生理指标包括支气管肺泡灌洗中获得的总细胞数增加、白细胞比例改变、总蛋白含量增加，以及以肺湿重与干重差值增加表示的肺水肿形成。与仅滴注标记物的对照大鼠（分别为1.8 +/- 0.4%、4.1 +/- 1.3%、20.0 +/- 3.8%）相比，气管内滴注右旋糖酐诱导了中度中性粒细胞侵入肺部，但对不同标记物经肺的通过率没有影响（BIgG为1.8 +/- 0.6%，BSA为3.5 +/- 1.2%，dDAVP为26.1 +/- 20.7%）。给予内毒素导致灌洗细胞计数和肺水肿明显更高，同时标记物经肺的通过率增加（分别为3.2 +/- 0.9%、22.0 +/- 6.1%、33.3 +/- 12.0%；P小于0.01 - P小于0.001）。当炎症最严重时，即给予铁蛋白后，标记物的通过率最高（分别为17.6 +/- 2.3%、60.0 +/- 6.7%、41.6 +/- 6.9%；P小于0.001），这导致灌洗细胞数量和蛋白质含量以及水肿形成明显升高。（摘要截于250字）
