Folkesson H G, Weström B R, Pierzynowski S G, Svendsen J, Karlsson B W
Department of Animal Physiology, University of Lund, Sweden.
Acta Physiol Scand. 1993 Feb;147(2):173-8. doi: 10.1111/j.1748-1716.1993.tb09486.x.
The passage via the lower respiratory tract of the macromolecule, bovine serum albumin (BSA, mol. wt = 67000) and the nonapeptide, 1-deaminocysteine-8-D-arginine vasopressin (dDAVP, mol. wt = 1067), was assessed for 240 h after intratracheal instillation in young, developing pigs. The plasma levels of immunoreactive BSA increased with time, reaching a maximum between 36-86 h after instillation, and the main portion of BSA in plasma appeared intact, as assessed by crossed immunoelectrophoresis. The dDAVP levels reached a maximum after 1-3 h. The time to reach maximal plasma levels increased with the age of the pig for BSA, while no such effect was observed for dDAVP. The total lung passage, as calculated by dividing the area under the plasma level-time curve obtained after intratracheal instillation with that obtained after intravenous administration (elimination curve), showed no significant differences with age for BSA (21.8 +/- 9.0% in the newborn, 15.1 +/- 8.0% in the 2-day-old and 16.1 +/- 4.2% in the 70-day-old pigs). For dDAVP, a significant decrease in the total passage was obtained with age, from 74.6 +/- 9.4% in the newborn pigs to 44.1 +/- 13.3%, in the 2-day-old pigs (P < 0.001), and to 23.6 +/- 7.1% (P < 0.01) in the 70-day-old pigs. The study showed that the marker molecules, BSA and dDAVP, passed over the porcine lung into the blood circulation in high amounts, apparently inversely related to their molecular size. The total lung passage of dDAVP, but not that of BSA, was affected by changes with age, indicating that these two markers might traverse the lung epithelium via different routes that are differently affected during postnatal development.
在幼龄发育中的猪气管内滴注大分子牛血清白蛋白（BSA，分子量 = 67000）和九肽1-脱氨基半胱氨酸-8-D-精氨酸加压素（dDAVP，分子量 = 1067）后，评估其通过下呼吸道的情况，持续240小时。免疫反应性BSA的血浆水平随时间升高，在滴注后36 - 86小时达到峰值，通过交叉免疫电泳评估，血浆中BSA的主要部分似乎保持完整。dDAVP水平在1 - 3小时后达到峰值。对于BSA，达到最大血浆水平的时间随猪的年龄增加而增加，而对于dDAVP未观察到这种影响。通过将气管内滴注后获得的血浆水平 - 时间曲线下面积除以静脉给药后获得的面积（消除曲线）计算得出的总肺通过率，对于BSA而言，不同年龄组之间无显著差异（新生猪为21.8±9.0%，2日龄猪为15.1±8.0%，70日龄猪为16.1±4.2%）。对于dDAVP，总通过率随年龄显著降低，新生猪为74.6±9.4%，2日龄猪为44.1±13.3%（P < 0.001），70日龄猪为23.6±7.1%（P < 0.01）。该研究表明，标记分子BSA和dDAVP大量通过猪肺进入血液循环，显然与它们的分子大小呈负相关。dDAVP的总肺通过率受年龄变化影响，而BSA不受影响，这表明这两种标记物可能通过不同途径穿过肺上皮，且在出生后发育过程中受到不同影响。
