使用微胶囊悬浮液对离心泵进行溶血评估。
Hemolysis evaluation of centrifugal pumps using microcapsule suspension.
作者信息
Ootaki Y, Kamohara K, Horvath D J, Massiello A, Golding L A R, Lukic B, Weiss W J, Maruyama O, Fukamachi K
机构信息
Department of Biomedical Engineering, Lerner Research Institute, The Cleveland Clinic, Cleveland, OH, USA.
出版信息
Int J Artif Organs. 2006 Dec;29(12):1185-9. doi: 10.1177/039139880602901212.
OBJECTIVES
Bovine and human blood has been widely used for in vitro hemolysis testing to evaluate centrifugal cardiac assist pumps. However, results from such tests are complicated by variations in the susceptibility of individual red blood cells to shear. The objective of this study was to evaluate the use of microcapsule suspension as an alternative to bovine or human blood for hemolysis testing.
METHODS
Microcapsule suspensions of 100 micro m maximal diameter (average 79.1 micro m) with a polyurethane membrane were used. Each microcapsule contained a leuco dye, which was used to measure "hemolysis" in the suspension after exposure to mechanical stress. Six centrifugal pumps were used to measure and compare the hemolysis values of microcapsule suspensions, bovine blood and human blood.
RESULTS
Correlations were significant between the hemolysis values measured using microcapsule suspensions and those using bovine blood (R = 0.965, p = 0.002) and human blood (R = 0.940, p = 0.005).
CONCLUSIONS
Microcapsule suspension can be successfully used instead of blood to compare the relative hemolytic performance of centrifugal blood pumps.
目的
牛血和人血已被广泛用于体外溶血试验，以评估离心式心脏辅助泵。然而，此类试验的结果因单个红细胞对剪切力敏感性的差异而变得复杂。本研究的目的是评估使用微胶囊悬浮液替代牛血或人血进行溶血试验的可行性。
方法
使用最大直径为100μm（平均79.1μm）、带有聚氨酯膜的微胶囊悬浮液。每个微胶囊含有一种无色染料，在暴露于机械应力后，该染料用于测量悬浮液中的“溶血”情况。使用六台离心泵测量并比较微胶囊悬浮液、牛血和人血的溶血值。
结果
使用微胶囊悬浮液测得的溶血值与使用牛血测得的溶血值之间存在显著相关性（R = 0.965，p = 0.002），与使用人血测得的溶血值之间也存在显著相关性（R = 0.940，p = 0.005）。
结论
微胶囊悬浮液可成功替代血液，用于比较离心式血泵的相对溶血性能。
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