Yamamoto K, Yamamoto M
Department of Cell Biology, Tokyo Metropolitan Institute of Gerontology, Japan.
Mutat Res. 1991 Mar-Nov;256(2-6):169-75. doi: 10.1016/0921-8734(91)90009-z.
The electrophoretic mobility of 13 human diploid cell strains, TIG-1, TIG-2, TIG-3, TIG-7, WI-38, IMR-90, MRC-5, MRC-9, TIG-1H, TIG-1L, TIG-2M, TIG-2B, and TIG-3S, which were established from different tissues of human embryos, was studied at different passages. The net negative surface charge of the cells was characteristic for each cell strain and decreased significantly during the in vitro aging of the cells. The decrease in the net negative charge of the cells correlated well with the decrease in cell density throughout the life span of the cells. A strict linear correlation between the electrophoretic mobility and the number of cells harvested at each passage was obtained for all the human diploid cell strains. Moreover, almost the same linear regression coefficient of the cells was obtained among these cell strains. Therefore, the net negative surface charge of human diploid cell strains could serve as a cell surface marker for in vitro cellular aging.
对从人类胚胎不同组织建立的13种人二倍体细胞株TIG-1、TIG-2、TIG-3、TIG-7、WI-38、IMR-90、MRC-5、MRC-9、TIG-1H、TIG-1L、TIG-2M、TIG-2B和TIG-3S在不同传代时的电泳迁移率进行了研究。细胞的净负表面电荷对每种细胞株来说都是特征性的，并且在细胞体外老化过程中显著降低。细胞净负电荷的减少与细胞整个生命周期中细胞密度的降低密切相关。对于所有的人二倍体细胞株，在电泳迁移率和每次传代收获的细胞数量之间获得了严格的线性相关性。此外，在这些细胞株之间获得了几乎相同的细胞线性回归系数。因此，人二倍体细胞株的净负表面电荷可以作为体外细胞老化的细胞表面标志物。
