Ganglioside changes during cell aging in human diploid fibroblast TIG-1.

Gangliosides of human diploid fibroblast, TIG-1, derived from fetal lung were analyzed during cell aging. The major gangliosides of TIG-1 cells were GM3 and GD3. During cell aging, GM3 and other gangliosides were decreased or below detection level. Lipid-bound sialic acid per mg protein was 2 micrograms at the early, middle and late passages, but between the middle and late passage it was twofold higher than the other passages.