Liang S H, Rieber P, Prewett M, Riethmüller G, Pletcher C, Hoxie J, Koprowski H, Herlyn D
Wistar Institute, Philadelphia, Pennsylvania.
Viral Immunol. 1991 Summer;4(2):83-90. doi: 10.1089/vim.1991.4.83.
Anti-idiotypic antibodies (Ab2) binding to the antigen-combining site of other antibodies may functionally and even structurally mimic antigen. Ab2 to antibodies directed against the lymphocyte CD4 receptor for human immunodeficiency virus type 1 (HIV-1) may mimic the receptor and therefore inhibit viral infectivity. We have produced Ab2 against monoclonal anti-CD4 receptor antibodies (Ab1). The Ab1 strongly inhibit HIV-1 binding to the receptor. Six monoclonal rat Ab2 and two polyclonal rabbit Ab2 were produced against the Ab1 MT151 and nine monoclonal Ab2 against the Ab1 OKT4A. These Ab2 bound only to Ab1 and not to a panel of nine unrelated murine monoclonal antibodies (MAbs). The Ab2 completely inhibited the binding of the homologous Ab1 to CD4-positive target cells, and recombinant soluble CD4 inhibited binding of Ab2 to Ab1. Thus, the Ab2 seemed to mimic the Ab1-binding site of the CD4 receptor, although the results of inhibition assays did not exclude steric hindrance of antibody-combining sites. However, none of the 17 Ab2 bound to gp120 of HIV-1 envelope or inhibited syncytia formation between cells infected and uninfected with HIV-1. These results suggest that the Ab2 do not mimic the HIV-1 binding site of the CD4 receptor. They further suggest that the Ab1 may not bind within the virus-binding site of the CD4 receptor.
与其他抗体的抗原结合位点相结合的抗独特型抗体（Ab2）在功能上甚至结构上可能模拟抗原。针对人免疫缺陷病毒1型（HIV-1）淋巴细胞CD4受体的抗体的Ab2可能模拟该受体，从而抑制病毒感染性。我们已经制备了针对单克隆抗CD4受体抗体（Ab1）的Ab2。Ab1强烈抑制HIV-1与受体的结合。针对Ab1 MT151制备了6种单克隆大鼠Ab2和2种多克隆兔Ab2，针对Ab1 OKT4A制备了9种单克隆Ab2。这些Ab2仅与Ab1结合，而不与一组9种不相关的鼠单克隆抗体（MAb）结合。Ab2完全抑制同源Ab1与CD4阳性靶细胞的结合，并且重组可溶性CD4抑制Ab2与Ab1的结合。因此，Ab2似乎模拟了CD4受体的Ab1结合位点，尽管抑制试验的结果并未排除抗体结合位点的空间位阻。然而，17种Ab2中没有一种与HIV-1包膜的gp120结合，也没有抑制感染和未感染HIV-1的细胞之间的合胞体形成。这些结果表明，Ab2不模拟CD4受体的HIV-1结合位点。它们进一步表明，Ab1可能不在CD4受体的病毒结合位点内结合。
