Sperlagh M, Hoxie J, Maruyama H, Stefano K, Gonzales-Scarano F, Prewett M, Liang S, Matsushita S, Herlyn D
Wistar Institute, Philadelphia, Pennsylvania.
Viral Immunol. 1994;7(2):61-9. doi: 10.1089/vim.1994.7.61.
Rabbit antiidiotypic antibodies (Ab2) were produced against anti-HIV-1 antibody 0.5 beta (Ab1), which binds to gp120 of HIV-1 and shows virus-neutralizing activity. The Ab2 bound specifically to the Ab1 and their binding to Ab2 was inhibited by a recombinant fragment of gp120 (PB1) or a peptide (residues 301-324 of gp120), both expressing the Ab1-defined epitope. The Ab2 induced in rats antiantiidiotypic antibodies (Ab3) that were Ab1-like in their binding reactivities to PB1, native gp120 or peptide and shared idiotopes with the Ab1. However, the Ab2 did not induce virus-neutralizing Ab3, probably a reflection of the low avidity of the Ab3 as compared to the Ab1.
