Gusakov Alexander V, Salanovich Tatyana N, Antonov Alexey I, Ustinov Boris B, Okunev Oleg N, Burlingame Richard, Emalfarb Mark, Baez Marco, Sinitsyn Arkady P
Department of Chemistry, M. V. Lomonosov Moscow State University, Moscow 119899, Russia.
Biotechnol Bioeng. 2007 Aug 1;97(5):1028-38. doi: 10.1002/bit.21329.
An extremely highly active cellobiohydrolase (CBH IIb or Cel6B) was isolated from Chrysosporium lucknowense UV18-25 culture filtrate. The CBH IIb demonstrated the highest ability for a deep degradation of crystalline cellulose amongst a few cellobiohydrolases tested, including C. lucknowense CBH Ia, Ib, IIa, and Trichoderma reesei CBH I and II. Using purified C. lucknowense enzymes (CBH Ia, Ib, and IIb; endoglucanases II and V; beta-glucosidase, xylanase II), artificial multienzyme mixtures were reconstituted, displaying an extremely high performance in a conversion of different cellulosic substrates (Avicel, cotton, pretreated Douglas fir wood) to glucose. These mixtures were much or notably more effective in hydrolysis of the cellulosic substrates than the crude multienzyme C. lucknowense preparation and other crude cellulase samples produced by T. reesei and Penicillium verruculosum. Highly active cellulases are a key factor in bioconversion of plant lignocellulosic biomass to ethanol as an alternative to fossil fuels.
从嗜热栖热放线菌UV18 - 25培养滤液中分离出一种活性极高的纤维二糖水解酶（CBH IIb或Cel6B）。在测试的几种纤维二糖水解酶中，包括嗜热栖热放线菌CBH Ia、Ib、IIa以及里氏木霉CBH I和II，CBH IIb对结晶纤维素的深度降解能力最强。使用纯化的嗜热栖热放线菌酶（CBH Ia、Ib和IIb；内切葡聚糖酶II和V；β - 葡萄糖苷酶、木聚糖酶II），重新构建了人工多酶混合物，该混合物在将不同纤维素底物（微晶纤维素、棉花、预处理的花旗松木材）转化为葡萄糖方面表现出极高的性能。这些混合物在纤维素底物水解方面比嗜热栖热放线菌粗多酶制剂以及里氏木霉和疣孢青霉产生的其他粗纤维素酶样品更有效得多或显著更有效。高活性纤维素酶是将植物木质纤维素生物质转化为乙醇以替代化石燃料的生物转化中的关键因素。
