Park Yong-Doo, Lyou You-Jeong, Yang Jun-Mo
Department of Dermatology, Sungkyunkwan University School of Medicine, Samsung Medical Center, Seoul, Korea.
Exp Dermatol. 2007 Feb;16(2):130-4. doi: 10.1111/j.1600-0625.2006.00524.x.
We conducted the proteomic studies to detect the dysregulated proteins in the atopic dermatitis (AD) proteome obtained from the patient-derived primary cultured fibroblasts. Acetaldehyde dehydrogenase 1 (ALDH1) was detected as being significantly down-regulated at the pH ranges of 6-9 and 4-7. The transcriptional levels of ALDH1, as detected by RT-PCR and real-time PCR, further confirmed the down-regulated phenomena for all the AD-fibroblasts (n = 20). The expression levels of ALDH1 in the whole skin tissue samples were further supported by the results of the primary cultured samples. These findings clearly demonstrate that ALDH1 can be a dermal biomarker for AD disease.
我们进行了蛋白质组学研究，以检测从患者来源的原代培养成纤维细胞获得的特应性皮炎（AD）蛋白质组中失调的蛋白质。在pH值范围为6 - 9和4 - 7时，检测到乙醛脱氢酶1（ALDH1）显著下调。通过逆转录聚合酶链反应（RT-PCR）和实时聚合酶链反应检测到的ALDH1转录水平，进一步证实了所有AD成纤维细胞（n = 20）的下调现象。原代培养样本的结果进一步支持了全皮肤组织样本中ALDH1的表达水平。这些发现清楚地表明，ALDH1可以作为AD疾病的皮肤生物标志物。
