Park Yong-Doo, Kim So-Yeon, Jang Hee-Sun, Seo Eun-Young, Namkung Jung-Hyun, Park Hyung-Seok, Cho Sang Yun, Paik Young-Ki, Yang Jun-Mo
Department of Dermatology, Sungkyunkwan University School of Medicine, Samsung Medical Center, Seoul, Korea.
Proteomics. 2004 Nov;4(11):3446-55. doi: 10.1002/pmic.200400998.
Atopic dermatitis (AD) is a chronic relapsing inflammatory skin disease typically characterized by a distribution of eczematous skin lesions with lichenification, pruritic excoriations, and dry skin with wide varieties of pathophysiologic aspects. Recently, AD was divided into extrinsic and intrinsic forms according to the presence or absence of an allergy. We investigated alterations in protein expression in primary cultured AD cells from the patient biopsy samples by two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight. In the primary cultured fibroblasts, we obtained 31 candidate proteins from the two-dimensional gel image analysis in which 18 proteins were up-regulated, eight proteins were down-regulated and five proteins were post-translationally modified. From these 2-DE results, we found several candidate genes matched proteomic expression patterns by semiquantitative reverse transcription PCR. Since the exact mechanism of atopic alterations in fibroblasts remains unknown, our results may provide new clues to aid in understanding AD.
特应性皮炎（AD）是一种慢性复发性炎症性皮肤病，其典型特征是湿疹性皮肤病变分布，伴有苔藓化、瘙痒性抓痕以及具有多种病理生理特征的干性皮肤。最近，AD根据是否存在过敏被分为外源性和内源性两种形式。我们通过二维凝胶电泳（2-DE）和基质辅助激光解吸/电离飞行时间质谱法，研究了来自患者活检样本的原代培养AD细胞中蛋白质表达的变化。在原代培养的成纤维细胞中，我们从二维凝胶图像分析中获得了31种候选蛋白，其中18种蛋白上调，8种蛋白下调，5种蛋白发生翻译后修饰。根据这些二维凝胶电泳结果，我们通过半定量逆转录PCR发现了几个与蛋白质组表达模式相匹配的候选基因。由于成纤维细胞中特应性改变的确切机制尚不清楚，我们的结果可能为理解AD提供新的线索。
