Kagawa Takatoshi, Suetsugu Noriyuki
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba 305-8572, Japan.
FEBS Lett. 2007 Feb 6;581(3):368-74. doi: 10.1016/j.febslet.2006.12.041. Epub 2007 Jan 11.
Chloroplast photoorientation in the green alga Mougeotia scalaris is controlled by blue and red light. The properties of the LOV domains of phototropin A and B were consistent with previous data of action spectra and photoreceptor lifetime for blue light-mediated photoorientation. The LOV domains of the neochromes did not bind flavin, while the domains of neochrome 2 contributed to multimer formation. The absorption spectra of the neochrome phytochrome photosensory domain with phytochromobilin were very similar to the action spectra for red light-induced photoorientation. These results indicate that phototropin and neochrome work as the blue and red photoreceptors involved in photoorientation.
绿藻中带形转板藻的叶绿体光取向受蓝光和红光控制。向光蛋白A和B的LOV结构域的特性与先前关于蓝光介导的光取向的作用光谱和光感受器寿命的数据一致。新色素的LOV结构域不结合黄素,而新色素2的结构域有助于多聚体形成。带有藻胆体的新色素光敏色素结构域的吸收光谱与红光诱导的光取向的作用光谱非常相似。这些结果表明,向光蛋白和新色素作为参与光取向的蓝光和红光光感受器发挥作用。