Hamid Q, Belvisi M G, Stretton D, Rohde J, Harmar A J, Barnes P J
Department of Pathology, National Heart and Lung Institute, London, UK.
Neuropeptides. 1991 Nov;20(3):145-50. doi: 10.1016/0143-4179(91)90123-z.
The neurons which synthesize tachykinins in the capsaicin-sensitive afferent nerves of the respiratory tract are largely localized to the nodose ganglia. Using a radiolabelled antisense cRNA probe constructed from cDNA for the major precursor of substance P and neurokinin A (beta-preprotachykinin: beta-PPT), we have localized specific mRNA for this peptide in neurons of the nodose ganglion of rat using in situ hybridization. 26% of neurons gave a positive hybridization signal, which was in agreement with the same proportion of cell bodies showing substance P-like immunoreactivity. The specificity of the hybridization was confirmed by the absence of labelling using RNase pre-treatment and a sense probe having the same sequence as beta-PPT mRNA. This approach may now allow the investigation of factors which regulate synthesis of tachykinins at a gene transcriptional level.
在呼吸道对辣椒素敏感的传入神经中合成速激肽的神经元大多定位于结状神经节。我们利用从P物质和神经激肽A的主要前体（β-前速激肽原：β-PPT）的cDNA构建的放射性标记反义cRNA探针，通过原位杂交在大鼠结状神经节的神经元中定位了该肽的特异性mRNA。26%的神经元给出了阳性杂交信号，这与显示P物质样免疫反应性的细胞体比例相同。通过核糖核酸酶预处理和与β-PPT mRNA序列相同的正义探针未出现标记，证实了杂交的特异性。这种方法现在可能有助于研究在基因转录水平调节速激肽合成的因素。
