Fedoruk-Wyszomirska Agnieszka, Wyszko Eliza, Giel-Pietraszuk Małgorzata, Barciszewska Mirosława Z, Barciszewski Jan
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
Int J Biol Macromol. 2007 Jun 1;41(1):30-5. doi: 10.1016/j.ijbiomac.2006.12.002. Epub 2006 Dec 17.
High hydrostatic pressure (HHP) technique was used to evaluate a mechanism of RNA hydrolysis with RNA. We showed that hammerhead ribozyme specifically cleaves RNA substrate at HHP in the absence of Mg(2+). A deoxyribozyme "10-23" was active in the same conditions. These results pointed out that the hydrolytic activity of nucleic acid depends on proper tertiary structure of a complex with a substrate. They prove that magnesium ion is not directly involved in catalysis process. On that basis we show the mechanism of RNA hydrolysis catalyzed with nucleic acids at HHP.
采用高静水压（HHP）技术评估RNA与RNA水解的机制。我们发现，在不存在Mg(2+)的情况下，锤头状核酶在高静水压下能特异性切割RNA底物。脱氧核酶“10 - 23”在相同条件下也具有活性。这些结果表明，核酸的水解活性取决于与底物形成的复合物的适当三级结构。它们证明镁离子并不直接参与催化过程。在此基础上，我们展示了核酸在高静水压下催化RNA水解的机制。
