单纯疱疹病毒1型被膜蛋白VP1/2和UL37与核内衣壳相关。
Herpes simplex virus type 1 tegument proteins VP1/2 and UL37 are associated with intranuclear capsids.
作者信息
Bucks Michelle A, O'Regan Kevin J, Murphy Michael A, Wills John W, Courtney Richard J
机构信息
Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.
出版信息
Virology. 2007 May 10;361(2):316-24. doi: 10.1016/j.virol.2006.11.031. Epub 2007 Jan 16.
The assembly of the tegument of herpes simplex virus type 1 (HSV-1) is a complex process that involves a number of events at various sites within virus-infected cells. Our studies focused on determining whether tegument proteins, VP1/2 and UL37, are added to capsids located within the nucleus. Capsids were isolated from the nuclear fraction of HSV-1-infected cells and purified by rate-zonal centrifugation to separate B capsids (containing the scaffold proteins and no viral DNA) and C capsids (containing DNA and no scaffold proteins). Western blot analyses of these capsids indicated that VP1/2 associated primarily with C capsids and UL37 associated with B and C capsids. The results demonstrate that at least two of the tegument proteins of HSV-1 are associated with capsids isolated from the nuclear fraction, and these capsid-tegument protein interactions may represent initial events of the tegumentation process.
单纯疱疹病毒1型（HSV-1）被膜的组装是一个复杂的过程，涉及病毒感染细胞内多个位点的一系列事件。我们的研究集中于确定被膜蛋白VP1/2和UL37是否添加到位于细胞核内的衣壳上。从HSV-1感染细胞的核部分分离出衣壳，并通过速率区带离心进行纯化，以分离B衣壳（含有支架蛋白且无病毒DNA）和C衣壳（含有DNA且无支架蛋白）。对这些衣壳的蛋白质印迹分析表明，VP1/2主要与C衣壳相关，而UL37与B衣壳和C衣壳相关。结果表明，HSV-1的至少两种被膜蛋白与从核部分分离出的衣壳相关，并且这些衣壳-被膜蛋白相互作用可能代表被膜形成过程的初始事件。
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