Naldinho-Souto Raquel, Browne Helena, Minson Tony
Division of Virology, Department of Pathology, University of Cambridge, UK.
J Virol. 2006 Mar;80(5):2582-4. doi: 10.1128/JVI.80.5.2582-2584.2006.
Immunogold electron microscopy was used to determine whether the tegument proteins VP13/14, VP22, and VP16 of herpes simplex virus type 1 (HSV1) are components of primary enveloped virions. Whereas VP13/14 and VP22 were not detected in virus particles in the perinuclear space and were present in only mature extracellular virions, VP16 was acquired prior to primary envelopment of the virus at the inner nuclear membrane. This finding highlights potential similarities and differences between HSV1 and the related alphaherpesvirus, pseudorabies virus, in which the homologues of all three of these tegument proteins are not incorporated into the virion until secondary envelopment.
免疫金电子显微镜技术被用于确定单纯疱疹病毒1型（HSV1）的被膜蛋白VP13/14、VP22和VP16是否为初级包膜病毒粒子的组成成分。虽然在核周空间的病毒粒子中未检测到VP13/14和VP22，且它们仅存在于成熟的细胞外病毒粒子中，但VP16在病毒于内核膜进行初级包膜形成之前就已获得。这一发现凸显了HSV1与相关的甲型疱疹病毒——伪狂犬病病毒之间潜在的异同，在伪狂犬病病毒中，这三种被膜蛋白的同源物直到次级包膜形成时才被纳入病毒粒子。
