Vamvakaki Vicky, Chaniotakis Nikos A
Laboratory of Analytical Chemistry, Department of Chemistry, University of Crete, Vassilika Vouton 71 003, Iraklion Crete, Greece.
Biosens Bioelectron. 2007 Jun 15;22(12):2848-53. doi: 10.1016/j.bios.2006.11.024. Epub 2007 Jan 16.
Monitoring of the organophosphorus pesticides dichlorvos and paraoxon at very low levels has been achieved with liposome-based nano-biosensors. The enzyme acetylcholinesterase was effectively stabilized within the internal nano-environment of the liposomes. Within the liposomes, the pH sensitive fluorescent indicator pyranine was also immobilized for the optical transduction of the enzymatic activity. Increasing amounts of pesticides lead to the decrease of the enzymatic activity for the hydrolysis of the acetylcholine and thus to a decrease in the fluorescent signal of the pH indicator. The decrease of the liposome biosensors signal is relative to the concentration of dichlorvos and paraoxon down to 10(-10)M levels. This biosensor system has been applied successfully to the detection of total toxicity in drinking water samples. Also a colorimetric screening device for pesticide analysis has been evaluated.
基于脂质体的纳米生物传感器已实现对极低水平有机磷农药敌敌畏和对氧磷的监测。乙酰胆碱酯酶在脂质体的内部纳米环境中得到有效稳定。在脂质体内，对pH敏感的荧光指示剂吡喃也被固定，用于酶活性的光学转导。农药含量增加会导致乙酰胆碱水解酶活性降低，进而使pH指示剂的荧光信号减弱。脂质体生物传感器信号的减弱与敌敌畏和对氧磷浓度低至10(-10)M水平相关。该生物传感器系统已成功应用于饮用水样品的总毒性检测。还评估了一种用于农药分析的比色筛选装置。
