Pearson P J, Walker M H
Cell Tissue Res. 1975 Dec 10;164(3):401-10. doi: 10.1007/BF00223017.
During spermatogenesis in the crab, Carcinus maenas, mitochondria in the developing spermatids degenerate. In close association with mitochondrial fragments in late spermatids, an elaborate lattice-like complex of fused membranes is formed and lies in a position adjacent to the acrosome. Mature sperm possess no mitochondria, but a whorl of membranes is present alongside the acrosome in the diffuse nucleus. To ascertain whether or not cytochrome c oxidase activity is lost as the mitochondria disintegrate, differentiating Carcinus maenas spermatids and mature sperm have been studied cytochemically. Cytochfome c oxidase activity was localised by means of the osmophilic compound 3,3'-diaminobenzidine (DAB). Cytochrome c oxidase activity is confined to the cristae of mitochondria in the testis. As spermatids mature most mitochondria are lost. A few mitochondrial fragments may be caught up within the lamellar complex. While they are recognisalbe as mitochondria they retain cytochrome c oxidase activity. The lamellar complex does not show this enzyme activity. These results therefore suggest that the mature sperm of Carcinus maenas do not contain the enzymes normally incorporated in the mitochondrial membrane, capable of oxidative phosphorylation.
在海蟹（Carcinus maenas）精子发生过程中，发育中的精子细胞内的线粒体退化。在晚期精子细胞中，与线粒体片段紧密相连的是一个由融合膜构成的精致的晶格状复合体，它位于顶体附近。成熟精子没有线粒体，但在弥散核中，顶体旁边有一圈膜。为了确定线粒体解体时细胞色素c氧化酶活性是否丧失，对处于分化阶段的海蟹精子细胞和成熟精子进行了细胞化学研究。细胞色素c氧化酶活性通过嗜锇性化合物3,3'-二氨基联苯胺（DAB）进行定位。细胞色素c氧化酶活性局限于睾丸中线粒体的嵴。随着精子细胞成熟，大多数线粒体丢失。一些线粒体片段可能会被卷入层状复合体中。当它们仍可被识别为线粒体时，会保留细胞色素c氧化酶活性。层状复合体不显示这种酶活性。因此，这些结果表明，海蟹的成熟精子不含有通常存在于线粒体内膜中、能够进行氧化磷酸化的酶。
