O'Brien J A, Walters R J, Sepúlveda F V
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, U.K.
Biochim Biophys Acta. 1991 Dec 9;1070(2):501-4. doi: 10.1016/0005-2736(91)90093-n.
Total crypt volume has been estimated by analysis of photographic images of intact viable crypts isolated from guinea-pig small intestine. Exposing these crypts to a hypotonic medium, led to transient swelling followed by regulatory volume decrease (RVD) in 12-20 min. RVD was blocked by inhibitors of K+ and Cl- conductance, suggesting that it occurs by activation of K+ and Cl- permeability pathways and loss of these ions.
通过对从豚鼠小肠分离出的完整活隐窝的照片图像进行分析，估算了隐窝的总体积。将这些隐窝暴露于低渗介质中，会导致短暂肿胀，随后在12 - 20分钟内出现调节性容积减小（RVD）。RVD被钾离子和氯离子电导抑制剂阻断，这表明它是通过激活钾离子和氯离子通透性途径以及这些离子的丢失而发生的。
