噬菌体R17被乙基化剂灭活：致死损伤

The inactivation of bacteriophage R17 by ethylating agents: the lethal lesions.

作者信息

Shooter K V, Howse R

出版信息

Chem Biol Interact. 1975 Dec;11(6):563-73. doi: 10.1016/0009-2797(75)90031-9.

DOI:10.1016/0009-2797(75)90031-9

Abstract

The biological inactivation of bacteriophage R17 by ethyl methanesulphonate (EMS) and N-ethyl-N-nitrosourea (ENUA) has been studied. At the mean lethal dose for the first compound 8 moles ethyl are bound/mole RNA and with the nitroso compound 3.5 moles ethyl are bound. Analysis of the amounts of the different ethylated derivatives formed shows that the toxicity of the sulphonate can be accounted for by the formation of 3-ethylcytosine, O6-ethylguanine, 1-ethyladenine and chain breaks produced on the hydrolysis of ethyl phosphotriesters. With the nitroso derivative on the other hand, the sum of chain breaks and of bases alkylated on a position involved in specific hydrogen bonding between base pairs only accounts for 65% of the observed toxicity. The possibility that 3-ethyladenine may constitute a lethal lesion is discussed.

摘要

研究了甲磺酸乙酯（EMS）和N-乙基-N-亚硝基脲（ENUA）对噬菌体R17的生物灭活作用。对于第一种化合物，在平均致死剂量下，每摩尔RNA结合8摩尔乙基；对于亚硝基化合物，每摩尔RNA结合3.5摩尔乙基。对形成的不同乙基化衍生物的量进行分析表明，磺酸盐的毒性可归因于3-乙基胞嘧啶、O6-乙基鸟嘌呤、1-乙基腺嘌呤的形成以及乙基磷酸三酯水解产生的链断裂。另一方面，对于亚硝基衍生物，链断裂和在碱基对之间特定氢键所涉及位置上烷基化的碱基之和仅占观察到的毒性的65%。讨论了3-乙基腺嘌呤可能构成致死损伤的可能性。

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