烷基化剂与噬菌体R17的反应。磷酸三酯形成的生物学效应。
The reaction of alkylating agents with bacteriophage R17. Biological effects of phosphotriester formation.
作者信息
Shooter K V, Howse R, Merrifield R K
出版信息
Biochem J. 1974 Feb;137(2):313-7. doi: 10.1042/bj1370313.
Abstract
The extent of biological inactivation and of the degradation of the RNA after reaction of bacteriophage R17 with ethyl methanesulphonate, isopropyl methanesulphonate and N-ethyl-N-nitrosourea was studied. Formation of breaks in the RNA chain probably results from hydrolysis of phosphotriesters formed in the alkylation reactions. Near neutral pH the ethyl and isopropyl phosphotriesters are sufficiently stable for the kinetics of the hydrolysis reaction to be followed. Results indicate that the rate of hydrolysis increases rapidly as the pH is raised. The evidence shows that a phosphotriester group does not itself constitute a lethal lesion. The extent of phosphotriester formation by the different agents is discussed in terms of reaction mechanism.
摘要
研究了噬菌体R17与甲磺酸乙酯、甲磺酸异丙酯和N-乙基-N-亚硝基脲反应后，RNA的生物失活程度和降解情况。RNA链断裂的形成可能是由于烷基化反应中形成的磷酸三酯水解所致。在接近中性pH值时，乙基和异丙基磷酸三酯足够稳定，可以跟踪水解反应的动力学。结果表明，随着pH值升高，水解速率迅速增加。证据表明，磷酸三酯基团本身并不构成致死损伤。根据反应机理讨论了不同试剂形成磷酸三酯的程度。
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