Rojas M V, Galanti N
Department of Cell Biology and Genetics, School of Medicine, University of Chile, Santiago.
FEBS Lett. 1991 Dec 16;295(1-3):31-4. doi: 10.1016/0014-5793(91)81377-k.
5-Azacytidine treatment of T. cruzi epimastigotes in culture induces active cell proliferation. This effect was detected as an increase in the cell number and [3H-methyl]thymidine incorporation into DNA. 5-Azacytidine does not alter other metabolic parameters. We have previously demonstrated that 5-azacytidine induces DNA hypomethylation in T. cruzi. Accordingly, we suggest that this chemical modification may be related to the control of T. cruzi cell division.
在培养物中用5-氮杂胞苷处理克氏锥虫无鞭毛体可诱导活跃的细胞增殖。这种效应表现为细胞数量增加以及[³H-甲基]胸苷掺入DNA。5-氮杂胞苷不会改变其他代谢参数。我们之前已证明5-氮杂胞苷可诱导克氏锥虫DNA低甲基化。因此，我们认为这种化学修饰可能与克氏锥虫细胞分裂的控制有关。
