Nelson P A, Lippke J A, Murcko M A, Rosborough S L, Peattie D A
Immunology, Molecular Biology and Molecular Modeling, Vertex Pharmaceuticals Incorporated, Cambridge, MA 02139-4211.
Gene. 1991 Dec 30;109(2):255-8. doi: 10.1016/0378-1119(91)90617-k.
A FKBP cDNA encoding murine FK506 binding protein (FKBP) has been cloned, and its complete nucleotide sequence has been determined. The open reading frame within the 1556-bp cDNA segment encodes an 108 amino acid (aa) protein that differs from the human FKBP by three aa and from the bovine FKBP by five aa. Molecular modeling of the protein places the aa substitutions at positions not directly involved in drug binding or interaction with the potential drug target protein, calcineurin A.
一个编码小鼠FK506结合蛋白（FKBP）的FKBP互补DNA（cDNA）已被克隆出来，并且其完整的核苷酸序列也已确定。在这个1556碱基对的cDNA片段中的开放阅读框编码一种由108个氨基酸组成的蛋白质，该蛋白质与人类FKBP有3个氨基酸的差异，与牛FKBP有5个氨基酸的差异。该蛋白质的分子建模显示，氨基酸替换发生在不直接参与药物结合或与潜在药物靶蛋白钙调神经磷酸酶A相互作用的位置。
