Wennström S, Sandström C, Claesson-Welsh L
Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden.
Growth Factors. 1991;4(3):197-208. doi: 10.3109/08977199109104816.
We have isolated and characterized a cDNA clone, phFGFR, encoding a human fibroblast growth factor (FGF) receptor. phFGFR contains an open reading frame which encodes an 820 amino acid polypeptide with three immunoglobulin-like domains in the extracellular part and an intracellular split tyrosine kinase domain. Transient expression in COS-1 cells and immunoprecipitation using an antiserum raised against a C-terminal peptide, gave rise to two components, representing mature (130 kDa) and precursor (115 kDa) forms of the phFGFR encoded polypeptide, which was denoted hFGFR-1. Crosslinking of iodinated acidic FGF (aFGF) and basic FGF (bFGF) to transiently expressing COS-1 cells revealed a major band of 95 kDa, which was competed for by both aFGF and bFGF. From Scatchard analyses, the Kd:s for binding of aFGF and bFGF to hFGFR-1 were estimated to 25 pM and 41 pM, respectively. Thus, phFGFR encodes a human FGF receptor with high affinity for both aFGF and bFGF.
我们已经分离并鉴定了一个编码人成纤维细胞生长因子（FGF）受体的cDNA克隆phFGFR。phFGFR包含一个开放阅读框，其编码一个820个氨基酸的多肽，该多肽在细胞外部分具有三个免疫球蛋白样结构域以及一个细胞内分裂酪氨酸激酶结构域。在COS-1细胞中进行瞬时表达，并使用针对C末端肽产生的抗血清进行免疫沉淀，产生了两个组分，代表phFGFR编码多肽的成熟形式（130 kDa）和前体形式（115 kDa），该多肽被命名为hFGFR-1。将碘化酸性FGF（aFGF）和碱性FGF（bFGF）与瞬时表达的COS-1细胞进行交联，显示出一条95 kDa的主要条带，aFGF和bFGF均可竞争结合该条带。通过Scatchard分析，估计aFGF和bFGF与hFGFR-1结合的Kd值分别为25 pM和41 pM。因此，phFGFR编码一种对aFGF和bFGF均具有高亲和力的人FGF受体。
