Foligne Benoit, Nutten Sophie, Grangette Corinne, Dennin Véronique, Goudercourt Denise, Poiret Sabine, Dewulf Joelle, Brassart Dominique, Mercenier Annick, Pot Bruno
Bactéries Lactiques and Immunité des Muqueuses, Institut Pasteur de Lille, 1 Rue du Professeur Calmette, Lille cedex F-59019, France.
World J Gastroenterol. 2007 Jan 14;13(2):236-43. doi: 10.3748/wjg.v13.i2.236.
To investigate the correlation between in vitro and in vivo immunomodulation potential of the probiotic strain and its ability to prevent experimental colitis in mice.
In vitro immunomodulation was assessed by measuring interleukin (IL)-12p70, IL-10, tumor necrosis factor alpha (TNFalpha) and interferon gamma (IFNgamma) release by human peripheral blood mononuclear cells (PBMCs) after 24 h stimulation with 13 live bacterial strains. A murine model of acute TNBS-colitis was next used to evaluate the prophylactic protective capacity of the same set of strains.
A strain-specific in vivo protection was observed. The strains displaying an in vitro potential to induce higher levels of the anti-inflammatory cytokine IL-10 and lower levels of the inflammatory cytokine IL-12, offered the best protection in the in vivo colitis model. In contrast, strains leading to a low IL-10/IL-12 cytokine ratio could not significantly attenuate colitis symptoms.
These results show that we could predict the in vivo protective capacity of the studied lactic acid bacteria (LAB) based on the cytokine profile we established in vitro. The PBMC-based assay we used may thus serve as a useful primary indicator to narrow down the number of candidate strains to be tested in murine models for their anti-inflammatory potential.
研究益生菌菌株的体外和体内免疫调节潜力与其预防小鼠实验性结肠炎能力之间的相关性。
通过测量13种活细菌菌株刺激人外周血单核细胞（PBMCs）24小时后白细胞介素（IL）-12p70、IL-10、肿瘤坏死因子α（TNFα）和干扰素γ（IFNγ）的释放来评估体外免疫调节。接下来使用急性TNBS结肠炎小鼠模型评估同一组菌株的预防性保护能力。
观察到菌株特异性的体内保护作用。在体外具有诱导更高水平抗炎细胞因子IL-10和更低水平炎性细胞因子IL-12潜力的菌株，在体内结肠炎模型中提供了最佳保护。相比之下，导致IL-10/IL-12细胞因子比率较低的菌株不能显著减轻结肠炎症状。
这些结果表明，我们可以根据在体外建立的细胞因子谱预测所研究的乳酸菌（LAB）的体内保护能力。因此，我们使用的基于PBMC的检测方法可能作为一种有用的初步指标，以缩小在小鼠模型中测试其抗炎潜力的候选菌株数量。
