Davies M E, Dingle J T, Pigott R, Power C, Sharma H
Strangeways Research Laboratory, Worts Causeway, Cambridge, U.K.
Connect Tissue Res. 1991;26(3):207-16. doi: 10.3109/03008209109152439.
Monoclonal antibodies have been used to demonstrate the induction of intercellular adhesion molecule 1 (ICAM-1) on chondrocytes in human articular cartilage. ICAM-1 was found not to be constitutively expressed but could be induced by exogenous interleukin 1 alpha(IL1- alpha) at concentrations ranging from 0.01 to 20 ng/ml during in vitro culture. Maximum expression was observed with 2-5ng/ml. In time-course experiments ICAM-1 was not expressed after 4h in culture with IL1 alpha. Expression was induced by 16h and was sustained for a minimum of 6 days in the continued presence of the cytokine. The endothelial leukocyte adhesion molecule (ELAM-1) was not expressed on chondrocytes and was not induced by IL1-alpha.
单克隆抗体已被用于证明人关节软骨中软骨细胞上细胞间黏附分子1（ICAM-1）的诱导情况。发现ICAM-1不是组成性表达的，但在体外培养期间，浓度范围为0.01至20 ng/ml的外源性白细胞介素1α（IL1-α）可诱导其表达。在2-5 ng/ml时观察到最大表达。在时间进程实验中，在与IL1α一起培养4小时后，ICAM-1未表达。16小时时诱导表达，并在细胞因子持续存在的情况下至少维持6天。内皮白细胞黏附分子（ELAM-1）在软骨细胞上未表达，也未被IL1-α诱导表达。
