Lapadula G, Iannone F, Zuccaro C, Grattagliano V, Covelli M, Patella V, Lo Bianco G, Pipitone V
Cattedra di Reumatologia, Università degli Studi Bari, Italy.
Clin Rheumatol. 1998;17(2):99-104. doi: 10.1007/BF01452253.
Cell-ECM (extracellular matrix) interactions are believed to play a key role in maintaining the normal structure of tissues such as cartilage. Cell surface adhesion molecules have been reported to mediate chondrocyte binding to ECM proteins in human normal cartilage but the behaviour of these molecules in human osteoarthritic cartilage is unknown. We studied receptor matrix proteins on freshly isolated chondrocytes obtained from 10 patients with osteoarthritis (OA). Chondrocytes were isolated by enzymatic digestion from three zones of the articular cartilage with a different degree of macroscopic and microscopic damage and chondrocyte phenotype was defined by flow cytometry. Chondrocytes strongly expressed beta1, integrin but not beta3 integrin. LFA-1 (CD18/CD11a) and ICAM-1 (CD54) antigens were almost undetectable. Interestingly, beta1 expression was significantly higher in the minimally damaged zone than in the zones with medium and maximum damage. These data show that beta1-integrin-mediated chondrocyte-ECM interactions decrease in osteoarthritic cartilage suggesting that perturbations of chondrocyte-matrix signalling occurs during OA.
细胞与细胞外基质（ECM）的相互作用被认为在维持诸如软骨等组织的正常结构中起着关键作用。据报道，细胞表面粘附分子介导人正常软骨中软骨细胞与ECM蛋白的结合，但这些分子在人骨关节炎软骨中的行为尚不清楚。我们研究了从10例骨关节炎（OA）患者新鲜分离的软骨细胞上的受体基质蛋白。通过酶消化从关节软骨的三个具有不同程度宏观和微观损伤的区域分离软骨细胞，并通过流式细胞术定义软骨细胞表型。软骨细胞强烈表达β1整合素，但不表达β3整合素。淋巴细胞功能相关抗原-1（LFA-1，CD18/CD11a）和细胞间粘附分子-1（ICAM-1，CD54）抗原几乎检测不到。有趣的是，β1表达在损伤最小的区域显著高于中度和最大损伤区域。这些数据表明，在骨关节炎软骨中，β1整合素介导的软骨细胞与ECM的相互作用减少，提示在骨关节炎期间软骨细胞-基质信号传导发生紊乱。
