Ferrer J F, Bhatt D M, Abt D A, Marshak R R, Baliga V L
Cornell Vet. 1975 Oct;65(4):527-42.
We report on an accurate, rapid and inexpensive test for the identification of animals infected with the Bovine C-type virus (BLV). The test involves the detection of serum antibodies to BLV using the immunofluorescent (IF) technique on acetone-fixed, infected cells. The specificity of the test was demonstrated by the fact that virus was found by electron microscopy in 90% of cattle showing positive reactions. In contrast, virus was not found despite extensive examination in antibody negative animals. Thus, the presence of IF antibody is an accurate indicator of current rather than past BLV infection. In order for the IF test to be specific it is of critical importance that the target cells used are infected only with BLV. BLV antibodies can also be detected by the immunoprecipitation (Ouchterlony) technique. However, a significant proportion of BLV infected animals showing positive reactions in the IF test failed to show precipitin antibodies to the virus. Likewise, BLV infection was demonstrated by both the IF test and electron microscopy in many animals with persistently normal levels of blood lymphocytes. Thus, neither the precipitin test nor the blood lymphocyte count (Bendixen's key) can be used to rule out BLV infection.
我们报告了一种用于鉴定感染牛C型病毒（BLV）动物的准确、快速且廉价的检测方法。该检测方法是在丙酮固定的感染细胞上，采用免疫荧光（IF）技术检测血清中针对BLV的抗体。通过电子显微镜在90%呈阳性反应的牛中发现病毒这一事实，证明了该检测方法的特异性。相比之下，尽管对抗体阴性的动物进行了广泛检查，但未发现病毒。因此，IF抗体的存在是当前而非过去BLV感染的准确指标。为使IF检测具有特异性，所使用的靶细胞仅感染BLV至关重要。BLV抗体也可通过免疫沉淀（欧氏）技术检测。然而，在IF检测中呈阳性反应的大量感染BLV的动物未能显示出针对该病毒的沉淀素抗体。同样，在许多血液淋巴细胞水平持续正常的动物中，IF检测和电子显微镜均证实了BLV感染。因此，沉淀素检测和血液淋巴细胞计数（本迪克森标准）均不能用于排除BLV感染。
