Cereda P M, Debiaggi M, Romero E
Microbiologica. 1984 Jan;7(1):107-12.
A new cell line, persistently infected with Bovine Leukemia Virus (BLV), was obtained by co-cultivation of lymphocytes from adult leukemic bovine with normal calf kidney cells. The presence of BLV was demonstrated directly in the lymphocytes and in the new cell line by electron microscopy, and by ELISA of cell extracts. An immunoperoxidase staining technique was used for detection of BLV-infected cells at each new passage of the line, in order to confirm the persistence of viral infection. Fixed preparations of infected cells were used to study human sera for the presence of specific antibody to BLV. Human sera were collected from different groups of patients, and a specific antibody response to BLV was detected in only 3 out of 33 sera from leukemic patients. No positivity was detected in control sera.
通过将成年白血病牛的淋巴细胞与正常小牛肾细胞共同培养，获得了一种持续感染牛白血病病毒（BLV）的新细胞系。通过电子显微镜以及细胞提取物的酶联免疫吸附测定（ELISA），直接在淋巴细胞和新细胞系中证实了BLV的存在。在该细胞系的每一次传代时，都使用免疫过氧化物酶染色技术检测BLV感染的细胞，以确认病毒感染的持续性。使用感染细胞的固定制剂研究人血清中是否存在针对BLV的特异性抗体。从不同组的患者中收集人血清，在33例白血病患者的血清中，仅在3例中检测到针对BLV的特异性抗体反应。在对照血清中未检测到阳性反应。
