Thurmond M C, Carter R L, Puhr D M, Burridge M J
Am J Vet Res. 1982 Jul;43(7):1152-5.
An estimated weighted-regression method was used to describe the decay of colostral bovine leukemia virus (BLV) antibodies in the calf, as measured by agar-gel immunodiffusion with glycoprotein antigen. The prediction equation, based on 473 observations from 130 animals, was log10 inverse titer = 1.29 -0.012 age (days). The half-life of BLV antibodies was estimated to be 25.8 days. Ages at which colostral antibodies were last detected were between 51 and 187 days. Normal limits of antibody decay were estimated and used to identify virus-induced active antibodies in calves during the colostral antibody period. Calves known to be infected were identified between 2 and 180 days of age, using 95% limits. Application of this procedure for the early serologic detection of BLV-infected calves in eradication or control programs is discussed.
采用估计加权回归法描述初乳中牛白血病病毒（BLV）抗体在犊牛体内的衰减情况，该衰减情况通过用糖蛋白抗原进行琼脂凝胶免疫扩散法测定。基于130只动物的473次观察结果得出的预测方程为：log10倒数滴度 = 1.29 - 0.012年龄（天）。BLV抗体的半衰期估计为25.8天。最后一次检测到初乳抗体的年龄在51至187天之间。估计了抗体衰减的正常范围，并用于识别初乳抗体期犊牛体内由病毒诱导产生的活性抗体。已知感染的犊牛在2至180日龄之间被识别出来，采用95%的界限值。本文讨论了该方法在根除或控制计划中对BLV感染犊牛进行早期血清学检测的应用。
