Eldeniz A U, Ozer F, Hadimli H H, Erganis O
Department of Endodontics, Faculty of Dentistry, University of Selcuk, Konya, Turkey.
Int Endod J. 2007 Feb;40(2):112-9. doi: 10.1111/j.1365-2591.2006.01190.x.
To compare the efficacy of a standard NaOCl irrigation procedure with that of Er,Cr:YSGG laser irradiation in contaminated root canals having small and large apical foramina.
Forty root canals of extracted central incisor teeth with straight roots were chosen so that their apical foramina just permitted the tip of a size 20-K file to pass through. The canals were then enlarged with files to size 60 and randomly divided into four groups of 10 teeth each. The apical foramina of one group were widened further so that the tip of a size 45-K file could just pass through. After sterilization, all roots were inoculated with Enterococcus faecalis for 48 h at 37 degrees C. The first group was used as a control, the second group was irrigated with 3% NaOCl solution for 15 min, and the last two groups having different sizes of apical foramina were irradiated with the Er,Cr:YSGG laser at output power from 0.5 W, with 20% air and water levels. The disinfecting efficacy of the groups was tested by collecting dentine chips from the inner canal walls of the specimens and counting viable E. faecalis on Mueller-Hinton agar plates.
The differences in the mean number of viable colonies between the control and laser groups were statistically significant (P < 0.05). The control specimens had the highest number of microorganisms (153 x 10(3) +/- 39 x 10(3)). Complete sterilization was achieved in the 3% NaOCl group. The mean colony forming units (CFU) values obtained after Er,Cr:YSGG laser irradiation were 6.6 x 10(3) CFU and 6.5 x 10(3) CFU in root canals having large and small apical foramina respectively.
In teeth with straight roots the Er,Cr:YSGG laser reduced the viable microbial population in root canals with small and large apical foramina but did not eradicate all bacteria. Three percent NaOCl inhibited the growth of E. faecalis and effectively sterilized all root canals.
比较标准次氯酸钠冲洗程序与铒铬:钇铝石榴石激光照射对根尖孔大小不同的污染根管的疗效。
选取40颗拔除的直根中央切牙的根管,使其根尖孔刚好能让20号K锉尖端通过。然后用锉将根管扩大至60号,并随机分为四组,每组10颗牙。将其中一组的根尖孔进一步扩大,使45号K锉尖端刚好能通过。消毒后,所有牙根在37℃下接种粪肠球菌48小时。第一组作为对照组,第二组用3%次氯酸钠溶液冲洗15分钟,最后两组根尖孔大小不同,用铒铬:钇铝石榴石激光以0.5W的输出功率照射,空气和水的比例为20%。通过从标本根管内壁收集牙本质碎屑并在穆勒-欣顿琼脂平板上计数存活的粪肠球菌来测试各组的消毒效果。
对照组和激光组之间存活菌落平均数的差异具有统计学意义(P<0.05)。对照标本中的微生物数量最多(153×10³±39×10³)。3%次氯酸钠组实现了完全消毒。铒铬:钇铝石榴石激光照射后,根尖孔大的根管和根尖孔小的根管中获得的平均菌落形成单位(CFU)值分别为6.6×10³CFU和6.5×10³CFU。
在直根牙中,铒铬:钇铝石榴石激光减少了根尖孔大小不同的根管中的活菌数量,但并未根除所有细菌。3%的次氯酸钠抑制了粪肠球菌的生长并有效消毒了所有根管。