Kushwah Jagriti, Mishra Rahul, Bhadauria Vishal
Department of Dentistry, Uttar Pradesh University of Medical Sciences, Saifai, Etawah, Uttar Pradesh, India.
Department of Orthodontics and Dentofacial Orthopaedics, IDEAS Dental College and Hospital, Gwalior, Madhya Pradesh, India.
Int J Clin Pediatr Dent. 2020 Nov-Dec;13(6):694-699. doi: 10.5005/jp-journals-10005-1860.
Evaluation and comparison of the antibacterial efficacy of sodium hypochlorite, ozonated water, diode laser, and diode laser in combination with ozonated water against .
One hundred and twenty extracted premolar teeth were sectioned at the cementoenamel junction and root canals were prepared using step-back technique with K-file up to #40. The teeth were arbitrarily allocated to four groups and stowed into brain heart infusion broth containing a microbial suspension of . Group I samples were irrigated with 3% sodium hypochlorite. Group II samples were irrigated with ozonated water. Group III samples were irrigated firstly by distilled water followed by laser irradiation. Group IV samples were irrigated by ozonated water followed by laser irradiation. After the treatment, the teeth were kept in vials containing 2 mL of nutrient broth. The vials were then incubated at 37°C for 24 h. Standard methods were then used to identify grown colonies.
Kruskal-Wallis test, Mann-Whitney test, Chi-square test, and Wilcoxon signed-rank test were used to measure the colony-forming units (CFUs) obtained at the end of the incubation period among the various groups for the evaluation of antibacterial efficacy of various disinfection protocols.
The highest mean CFU/mL is recorded in group II (with ozonated water) followed by group I (with sodium hypochlorite), group III (with a laser), and the least mean CFU/mL is seen in group IV (with laser and ozonated water). The variation in CFU/mL among the three groups is statistically significant ( < 0.05).
The outcome of this study reveals that 980 nm diode laser along with ozonated water when used together can eliminate and disinfect the root canals with .
Kushwah J, Mishra R, Bhadauria V. Antibacterial Efficacy of Sodium Hypochlorite, Ozonated Water, and 980 nm Diode Laser Used for Disinfection of Root Canal against : A Microbiological Study. Int J Clin Pediatr Dent 2020;13(6):694-699.
评估和比较次氯酸钠、臭氧水、二极管激光以及二极管激光联合臭氧水对……的抗菌效果。
将120颗拔除的前磨牙在牙骨质釉质界处截断，使用K锉采用逐步后退技术预备根管至#40。将牙齿随机分为四组，并置于含有……微生物悬液的脑心浸液肉汤中。第一组样本用3%次氯酸钠冲洗。第二组样本用臭氧水冲洗。第三组样本先用蒸馏水冲洗，然后进行激光照射。第四组样本先用臭氧水冲洗，然后进行激光照射。处理后，将牙齿保存在含有2 mL营养肉汤的小瓶中。然后将小瓶在37°C下孵育24小时。接着使用标准方法鉴定生长的菌落。
采用Kruskal-Wallis检验、Mann-Whitney检验、卡方检验和Wilcoxon符号秩检验来测量孵育期结束时各组获得的菌落形成单位（CFU），以评估各种消毒方案的抗菌效果。
第二组（使用臭氧水）记录的平均CFU/mL最高，其次是第一组（使用次氯酸钠）、第三组（使用激光），第四组（使用激光和臭氧水）的平均CFU/mL最少。三组之间CFU/mL的差异具有统计学意义（<0.05）。
本研究结果表明，980 nm二极管激光与臭氧水联合使用时可消除并消毒患有……的根管。
Kushwah J, Mishra R, Bhadauria V. 次氯酸钠、臭氧水和980 nm二极管激光用于根管消毒对……的抗菌效果：一项微生物学研究。《国际临床儿科牙科学杂志》2020；13(6):694 - 699。
