Silva Garcez Aguinaldo, Núñez Silvia Cristina, Lage-Marques José Luís, Jorge Antonio Olavo Cardoso, Ribeiro Martha Simões
Center for Lasers and Applications, IPEN-CNEN/SP, São Paulo, Brazil.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Oct;102(4):e93-8. doi: 10.1016/j.tripleo.2006.02.015. Epub 2006 Aug 10.
To investigate the action of a red laser associated with a photosensitizer on the reduction of Enterococcus faecalis in dental root canal in vitro.
Thirty prepared teeth with single canals were contaminated. The chemical group was irrigated with 0.5% NaOCl and left flooded for 30 minutes. In the laser group, a paste-based photosensitizer was maintained in the root canals for 5 minutes, and then irradiated with a laser at 685 nm using an optical fiber for an E of 1.8 J during 3 minutes. After treatment, the canal content was collected, serially diluted, and cultured to determine the number of colony-forming units.
Photosensitizer alone or laser alone did not have any bactericidal effect. Chemical solution reduced viable bacteria in 93.25%. Laser photosensitization resulted in a reduction of 99.2%, a significantly higher bacterial reduction than NaOCl.
Laser photosensitization was effective for reducing E. faecalis in root canals and could be an adjunct to endodontic treatment.
体外研究红色激光联合光敏剂对减少牙根管内粪肠球菌的作用。
选取30颗单根管预备好的牙齿进行污染处理。化学药物组用0.5%次氯酸钠冲洗并保持浸泡30分钟。激光组将糊剂型光敏剂置于根管内5分钟，然后使用光纤以685nm波长的激光照射3分钟，能量为1.8J。处理后，收集根管内容物，进行系列稀释并培养，以确定菌落形成单位的数量。
单独使用光敏剂或单独使用激光均无杀菌作用。化学溶液可使活菌减少93.25%。激光光敏化导致细菌减少99.2%，细菌减少率显著高于次氯酸钠。
激光光敏化对减少根管内粪肠球菌有效，可作为牙髓病治疗的辅助手段。
