通过基质辅助激光解吸电离飞行时间质谱突变检测监测慢性感染中的丙型肝炎病毒准种
Monitoring of hepatitis C virus quasispecies in chronic infection by matrix-assisted laser desorption ionization-time of flight mass spectrometry mutation detection.
作者信息
Yea Carmen, Bukh Jens, Ayers Melissa, Roberts Eve, Krajden Mel, Tellier Raymond
机构信息
Metabolism Research Program, The Hospital for Sick Children, Toronto, Canada.
出版信息
J Clin Microbiol. 2007 Mar;45(3):1053-7. doi: 10.1128/JCM.02512-06. Epub 2007 Jan 17.
Abstract
Using both a mass spectrometry-based method and the classical method of cloning and sequencing, we demonstrated weekly changes in the hypervariable region 1 quasispecies of a chimpanzee infected with an infectious clone, coinciding with neutralizing antibody emergence. We also used the mass spectrometry method in the clinical follow-up of a chronically infected patient over a 5-year period.
摘要
我们使用基于质谱的方法以及传统的克隆和测序方法，证明了感染感染性克隆的黑猩猩高变区1准种的每周变化，这与中和抗体的出现相吻合。我们还在一名慢性感染患者的5年临床随访中使用了质谱方法。
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