Gumurdulu Derya, Erdogan Seyda, Kayaselcuk Fazilet, Seydaoglu Gulsah, Parsak Cem-K, Demircan Orhan, Tuncer Ilhan
Department of Pathology of Affiliated Hospital, Faculty of Medicine, Cukurova University, Adana 01330, Turkey.
World J Gastroenterol. 2007 Jan 21;13(3):426-31. doi: 10.3748/wjg.v13.i3.426.
To investigate the expression of Cyclooxygenase-2 (COX-2), proliferating cell nuclear antigen (PCNA), Ki-67 and p53 in gastrointestinal stromal tumors (GISTs) and its relationship with histopathological parameters.
Twenty-five GISTs were examined by light microscopy and immunohistochemistry. c-kit, CD34, SMA, S-100 protein, COX-2, PCNA, Ki-67 and p53 were detected immunohistochemically and the relationship was evaluated among histopathologic parameters such as mitotic index (MI), tumor grade, tumor size, COX-2, PCNA, Ki-67 and p53.
COX-2 protein expression was found in 19 of 25 (76%) of the tumors, and expression was noted in the cytoplasm of the tumor cells. p53 was significantly related to MI and tumor grade but no relationship was found between COX-2, proliferation markers and MI, tumor grade and tumor size.
COX-2 is expressed in most GISTs and it may play an important role in the proliferation and progression of these tumors or a useful marker to identify GIST. Although immunohistochemical assessment of p53 can be used for distinguishing the risk groups of GISTs, tumor size and mitotic rate should be considered at the same time.
研究环氧化酶-2（COX-2）、增殖细胞核抗原（PCNA）、Ki-67和p53在胃肠道间质瘤（GISTs）中的表达及其与组织病理学参数的关系。
对25例GISTs进行光学显微镜和免疫组织化学检查。采用免疫组织化学方法检测c-kit、CD34、平滑肌肌动蛋白（SMA）、S-100蛋白、COX-2、PCNA、Ki-67和p53，并评估有丝分裂指数（MI）、肿瘤分级、肿瘤大小、COX-2、PCNA、Ki-67和p53等组织病理学参数之间的关系。
25例肿瘤中有19例（76%）检测到COX-2蛋白表达，且在肿瘤细胞的细胞质中可见表达。p53与MI和肿瘤分级显著相关，但未发现COX-2、增殖标志物与MI、肿瘤分级及肿瘤大小之间存在相关性。
COX-2在大多数GISTs中表达，可能在这些肿瘤的增殖和进展中起重要作用，或是识别GIST的有用标志物。虽然p53的免疫组织化学评估可用于区分GISTs的风险组，但同时应考虑肿瘤大小和有丝分裂率。
