Seeni Azman, Baig Atif Amin, Murtey Mogana Das
Department of Toxicology, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, Kepala Batas 13200, Penang, Malaysia.
Advanced Material Research Center, SIRIM Industrial Research, SIRIM Berhad, Kulim Hi-Tech Park, Kulim 09000, Kedah, Malaysia.
Anal Cell Pathol (Amst). 2025 Mar 13;2025:4029625. doi: 10.1155/ancp/4029625. eCollection 2025.
Benign prostate hyperplasia (BPH) is common in elderly men. Previously, paddy waste (both husk and straw) reportedly had chemopreventive potential. The main aim of this study was to explore the chemopreventive properties of paddy waste against prostate disease. This study determines the antiproliferative activity of the paddy waste product in spontaneously hypertensive rats (SHRs). Aqueous methanol extracts of paddy husk and straw were administered to SHRs for 17 weeks via drinking water, with no observed toxicity on dietary intake, body weight, liver, or kidney. The study used 18 male SHRs to model primary hypertension and 6 male Wistar Kyoto (WKY) rats as normotensive controls. The SHRs were divided into three groups: control ( = 6), paddy husk treated ( = 6, 15 mg/kg), and paddy straw treated ( = 6, 15 mg/kg), with treatment delivered in drinking water. It managed to reduce blood pressure (72.0 mmHg; < 0.01) and the size of the ventral prostate to around 0.05% ( < 0.01). Histological analysis revealed antiproliferative signs such as a reduction in the number of acini (7.50; < 0.01), epithelial height (10.55 µm; < 0.01), and epithelial acinar area (18.17%; < 0.01). Aqueous methanol extracts have arrested the cell cycle by downregulating ( < 0.01) proliferative marker, Ki-67, and proliferating cell nuclear antigen (PCNA). Prostate cell growth is arrested by downregulation of androgen receptor (AR) which inhibited AR mRNA transcription (RTPCR analysis) and induced cell cycle arrest at the S phase through p27 and cyclin E2 (western blot analysis). In conclusion, paddy waste product especially husk is a better chemopreventive agent against prostate disease.
良性前列腺增生（BPH）在老年男性中很常见。此前，据报道稻壳废料（包括稻壳和稻草）具有化学预防潜力。本研究的主要目的是探索稻壳废料对前列腺疾病的化学预防特性。本研究确定了稻壳废料产品对自发性高血压大鼠（SHR）的抗增殖活性。将稻壳和稻草的甲醇水提取物通过饮用水给予SHR 17周，未观察到对饮食摄入、体重、肝脏或肾脏的毒性。该研究使用18只雄性SHR作为原发性高血压模型，6只雄性Wistar Kyoto（WKY）大鼠作为正常血压对照。SHR被分为三组：对照组（=6）、稻壳处理组（=6，15mg/kg）和稻草处理组（=6，15mg/kg），通过饮用水进行处理。它成功降低了血压（72.0mmHg；<0.01），腹侧前列腺大小减小到约0.05%（<0.01）。组织学分析显示出抗增殖迹象，如腺泡数量减少（7.50；<0.01）、上皮高度降低（10.55μm；<0.01）和上皮腺泡面积减小（18.17%；<0.01）。甲醇水提取物通过下调（<0.01）增殖标志物Ki-67和增殖细胞核抗原（PCNA）使细胞周期停滞。前列腺细胞生长通过下调雄激素受体（AR）而停滞，AR抑制AR mRNA转录（RTPCR分析），并通过p27和细胞周期蛋白E2诱导细胞周期在S期停滞（蛋白质印迹分析）。总之，稻壳废料产品尤其是稻壳是一种更好的预防前列腺疾病的化学预防剂。
