多种去污剂提取伯氏疏螺旋体和赫氏疏螺旋体蛋白质的比较能力。

Comparative ability of various detergents to extract proteins of Borrelia burgdorferi and Borrelia hermsii.

作者信息

Sambri V, Cevenini R

机构信息

Institute of Microbiology, University of Bologna, St. Orsola Hospital, Italy.

出版信息

Microbiologica. 1991 Oct;14(4):307-13.

PMID:1723136

Abstract

Borrelia burgdorferi and Borrelia hermsii were treated with the following detergents: sodium dodecyl sulphate (SDS), N-lauryl sarcosine (Sarkosyl) and Triton X-100, and the soluble and insoluble fractions obtained after each detergent treatment were examined by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Sarkosyl as well as SDS solubilized all Borrelia proteins, whereas Triton X-100 treatment selectively solubilized the majority of other borrelial proteins, leaving two proteins of 41 kDa and 66 kDa in Borrelia burgdorferi and one protein of 39 kDa in Borrelia hermsii associated with the Triton X-100 insoluble fraction.

摘要

用以下去污剂处理伯氏疏螺旋体和赫氏疏螺旋体

十二烷基硫酸钠（SDS）、N-月桂酰肌氨酸（ Sarkosyl）和 Triton X-100，每种去污剂处理后得到的可溶性和不溶性组分通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳进行检测。Sarkosyl和 SDS 均可溶解所有疏螺旋体蛋白，而 Triton X-100 处理则选择性地溶解了大多数其他疏螺旋体蛋白，在伯氏疏螺旋体中留下了两种分子量分别为 41 kDa 和 66 kDa 的蛋白，在赫氏疏螺旋体中留下了一种分子量为 39 kDa 的蛋白与 Triton X-100 不溶性组分相关联。